Gene drives for pest control: a recap
Authorship
D.F.F.
Biotechnology Degree (2nd Ed. )
D.F.F.
Biotechnology Degree (2nd Ed. )
Defense date
09.11.2025 09:30
09.11.2025 09:30
Summary
Pest control remains one of the main challenges for public health, agriculture, and biodi-versity conservation. In recent decades, the development of genetic technologies such as gene drives has opened new possibilities for the specific and long-term control of tar-get species. This work aims to provide a bibliographic review and critical analysis of pest control methods based on gene drive technology, comparing them to conventional strat-egies and evaluating their environmental and social implications. The study examines the main molecular mechanisms employed, the types of gene drives developed to date, their technical limitations, and the ecological risks associated with their release. It also compares their advantages and drawbacks with traditional methods such as pesticides and identifies key ethical, regulatory, and scientific challenges that affect their future implementation. The results of this review suggest that although gene drives offer significant potential as a control tool, their use entails unresolved technical challenges and considerable ecolog-ical uncertainty. It is concluded that their application should proceed under robust inter-national governance frameworks, guided by scientific evidence, the precautionary princi-ple, and active public engagement.
Pest control remains one of the main challenges for public health, agriculture, and biodi-versity conservation. In recent decades, the development of genetic technologies such as gene drives has opened new possibilities for the specific and long-term control of tar-get species. This work aims to provide a bibliographic review and critical analysis of pest control methods based on gene drive technology, comparing them to conventional strat-egies and evaluating their environmental and social implications. The study examines the main molecular mechanisms employed, the types of gene drives developed to date, their technical limitations, and the ecological risks associated with their release. It also compares their advantages and drawbacks with traditional methods such as pesticides and identifies key ethical, regulatory, and scientific challenges that affect their future implementation. The results of this review suggest that although gene drives offer significant potential as a control tool, their use entails unresolved technical challenges and considerable ecolog-ical uncertainty. It is concluded that their application should proceed under robust inter-national governance frameworks, guided by scientific evidence, the precautionary princi-ple, and active public engagement.
Direction
GARCIA SUAREZ, CARLOS (Tutorships)
GARCIA SUAREZ, CARLOS (Tutorships)
Court
LEIRO VIDAL, JOSE MANUEL (Chairman)
PARGA MARTIN, JUAN ANDRES (Secretary)
MAURICIO IGLESIAS, MIGUEL (Member)
LEIRO VIDAL, JOSE MANUEL (Chairman)
PARGA MARTIN, JUAN ANDRES (Secretary)
MAURICIO IGLESIAS, MIGUEL (Member)
Study of the type III secretion system (T3SS) in the bivalve pathogen Vibrio europaeus
Authorship
P.G.V.
Bachelor of Biology
P.G.V.
Bachelor of Biology
Defense date
09.12.2025 09:00
09.12.2025 09:00
Summary
Infection caused by bacteria of the genus Vibrio can affect various marine organisms, including bivalves farmed in aquaculture. Among the bacterial pathogens that affect the latter, the species Vibrio europaeus stands out, due to the wide range of hosts it is capable of infecting. Bacterial secretion systems play an important role in the virulence of these pathogens. The type III secretion system characteristic of Gram-negative bacteria acts as a virulence factor by being responsible for the introduction of effector proteins into the host cell. In this study, the role of the type III secretion system in Vibrio europaeus by comparing a defective strain with the wild strain and examined the influence of temperature and salinity on its function. To this end, the phenotypic and physiological behaviour and virulence of both strains were studied in different experiments. The results showed that the secretory system analysed conditions the growth of Vibrio europaeus at 25ºC, as well as its lipolytic activity, its ability to form biofilm and its virulence. They also demonstrated the influence of temperature on the growth and gene expression of this species and the possible existence of functional compensation between virulence factors.
Infection caused by bacteria of the genus Vibrio can affect various marine organisms, including bivalves farmed in aquaculture. Among the bacterial pathogens that affect the latter, the species Vibrio europaeus stands out, due to the wide range of hosts it is capable of infecting. Bacterial secretion systems play an important role in the virulence of these pathogens. The type III secretion system characteristic of Gram-negative bacteria acts as a virulence factor by being responsible for the introduction of effector proteins into the host cell. In this study, the role of the type III secretion system in Vibrio europaeus by comparing a defective strain with the wild strain and examined the influence of temperature and salinity on its function. To this end, the phenotypic and physiological behaviour and virulence of both strains were studied in different experiments. The results showed that the secretory system analysed conditions the growth of Vibrio europaeus at 25ºC, as well as its lipolytic activity, its ability to form biofilm and its virulence. They also demonstrated the influence of temperature on the growth and gene expression of this species and the possible existence of functional compensation between virulence factors.
Direction
DUBERT PEREZ, JAVIER (Tutorships)
Vázquez Barca, Alba (Co-tutorships)
DUBERT PEREZ, JAVIER (Tutorships)
Vázquez Barca, Alba (Co-tutorships)
Court
MUÑOZ CREGO, MARIA ANGELES (Chairman)
ROMERO BERNARDEZ, MANUEL (Secretary)
SALGADO CASTRO, FRANCISCO JAVIER (Member)
MUÑOZ CREGO, MARIA ANGELES (Chairman)
ROMERO BERNARDEZ, MANUEL (Secretary)
SALGADO CASTRO, FRANCISCO JAVIER (Member)
Ecophysiological aspects and impacts of the invasive plant Tradescantia fluminensis: A review paper
Authorship
M.G.V.
Double bachelor degree in Chemistry and Biology
M.G.V.
Double bachelor degree in Chemistry and Biology
Defense date
09.11.2025 10:00
09.11.2025 10:00
Summary
Tradescantia fluminensis, commonly known as wandering Jew or inch plant, is a member of the Commelinaceae family and originates from South America. It is a perennial, herbaceous species with highly branched stems, reaching heights between 30 and 60 centimeters. It has been introduced to almost every country in the world for ornamental purposes and remains very common in gardening today. Due to this artificial geographic expansion, it has become problematic in many countries because of its invasive nature and demographic expansion, growing very rapidly in shady and moist environments. This species primarily establishes itself in riparian forests and poses a significant threat to these habitats in places like Australia, New Zealand, and the United States, as well as in Mediterranean European countries such as Portugal, Italy, and Spain. In Spain, Galicia is one of the most affected areas, along with the Cantabrian and Mediterranean coasts and the Canary Islands. This paper presents a literature review of documents referring to T. fluminensis, aiming to select the most relevant and, when possible, the most recent information regarding its impact on biodiversity, control or eradication methods that can be applied, and the potential benefits that can be obtained from this species. The reviewed works highlight that T. fluminensis affects both flora and fauna, reducing the diversity and cover of native vegetation and altering the abundance and diversity of invertebrates. From a management perspective, some authors emphasize that biological control methods are the most advisable, although herbicides such as triclopyr can also be used. Regarding the potential uses of the plant, paper production and the extraction of antioxidant compounds are considered, although further exploration of management strategies is needed that focus on making use of plant waste generated through mechanical control methods such as manual removal.
Tradescantia fluminensis, commonly known as wandering Jew or inch plant, is a member of the Commelinaceae family and originates from South America. It is a perennial, herbaceous species with highly branched stems, reaching heights between 30 and 60 centimeters. It has been introduced to almost every country in the world for ornamental purposes and remains very common in gardening today. Due to this artificial geographic expansion, it has become problematic in many countries because of its invasive nature and demographic expansion, growing very rapidly in shady and moist environments. This species primarily establishes itself in riparian forests and poses a significant threat to these habitats in places like Australia, New Zealand, and the United States, as well as in Mediterranean European countries such as Portugal, Italy, and Spain. In Spain, Galicia is one of the most affected areas, along with the Cantabrian and Mediterranean coasts and the Canary Islands. This paper presents a literature review of documents referring to T. fluminensis, aiming to select the most relevant and, when possible, the most recent information regarding its impact on biodiversity, control or eradication methods that can be applied, and the potential benefits that can be obtained from this species. The reviewed works highlight that T. fluminensis affects both flora and fauna, reducing the diversity and cover of native vegetation and altering the abundance and diversity of invertebrates. From a management perspective, some authors emphasize that biological control methods are the most advisable, although herbicides such as triclopyr can also be used. Regarding the potential uses of the plant, paper production and the extraction of antioxidant compounds are considered, although further exploration of management strategies is needed that focus on making use of plant waste generated through mechanical control methods such as manual removal.
Direction
RETUERTO FRANCO, JOSE CARLOS RUBÉN (Tutorships)
RODRIGUEZ PARRA, JONATAN (Co-tutorships)
RETUERTO FRANCO, JOSE CARLOS RUBÉN (Tutorships)
RODRIGUEZ PARRA, JONATAN (Co-tutorships)
Court
PRIETO LAMAS, BEATRIZ LORETO (Chairman)
BASELGA FRAGA, ANDRES (Secretary)
AMIGO VAZQUEZ, FRANCISCO JAVIER (Member)
PRIETO LAMAS, BEATRIZ LORETO (Chairman)
BASELGA FRAGA, ANDRES (Secretary)
AMIGO VAZQUEZ, FRANCISCO JAVIER (Member)
Electrochemical synthesis and structural study of metal complexes with polydentate ligands
Authorship
M.G.V.
Double bachelor degree in Chemistry and Biology
M.G.V.
Double bachelor degree in Chemistry and Biology
Defense date
09.18.2025 16:00
09.18.2025 16:00
Summary
This work describes the synthesis of cobalt, nickel, and zinc complexes with the ligand (Z)-N-isonicotinoylpicolinohydrazonamide (HLA), as well as a lead complex with the ligand (Z)-N-benzoylpicolinohydrazonamide (HLB), using an electrochemical synthesis method. This procedure has proven to be a highly effective technique, allowing the formation of complexes with high purity and an electrochemical efficiency of 0.5, which demonstrates that the metal is oxidized to M(II) in all the compounds. All complexes were characterized using the following experimental analytical techniques: elemental analysis, infrared spectroscopy, 1H and 13C nuclear magnetic resonance and X-ray diffraction.
This work describes the synthesis of cobalt, nickel, and zinc complexes with the ligand (Z)-N-isonicotinoylpicolinohydrazonamide (HLA), as well as a lead complex with the ligand (Z)-N-benzoylpicolinohydrazonamide (HLB), using an electrochemical synthesis method. This procedure has proven to be a highly effective technique, allowing the formation of complexes with high purity and an electrochemical efficiency of 0.5, which demonstrates that the metal is oxidized to M(II) in all the compounds. All complexes were characterized using the following experimental analytical techniques: elemental analysis, infrared spectroscopy, 1H and 13C nuclear magnetic resonance and X-ray diffraction.
Direction
LABISBAL VIQUEIRA, MARIA ELENA (Tutorships)
García Santos, María Isabel (Co-tutorships)
LABISBAL VIQUEIRA, MARIA ELENA (Tutorships)
García Santos, María Isabel (Co-tutorships)
Court
GONZALEZ BELLO, CONCEPCION (Chairman)
SECO CASTRO, JOSÉ MANUEL (Secretary)
VAZQUEZ LOPEZ, MIGUEL (Member)
GONZALEZ BELLO, CONCEPCION (Chairman)
SECO CASTRO, JOSÉ MANUEL (Secretary)
VAZQUEZ LOPEZ, MIGUEL (Member)
Study of the effect of obestatin treatment in an in vivo model of murine pancreatic ductal adenocarcinoma.
Authorship
L.G.V.
Bachelor of Biology
L.G.V.
Bachelor of Biology
Defense date
09.12.2025 09:00
09.12.2025 09:00
Summary
This study investigates the potential therapeutic role of obestatin in the context of pancreatic ductal adenocarcinoma, using an orthotopic mouse model based on the KPC FC1245 cell line. Based on the hypothesis that obestatin could modulate key processes in the tumor microenvironment, the objectives were to analyze its impact on parameters such as apoptosis, autophagy, metastasis, inflammation, hypoxia, senescence, fibrogenesis, and immune response. The methodology employed included the implantation of tumor cells in C57BL/6 mice, treatment with obestatin for fourteen days, and subsequent protein analysis using immunoblot techniques. The main results show that obestatin reduces the expression of senescence markers (p21 and p16) and increases that of a metastasis suppressor (nm23-H1), without significantly altering other parameters such as inflammation or GPR39 receptor expression. These findings indicate a limited but selective effect of obestatin on certain processes associated with tumor progression, suggesting that it could contribute to a less aggressive microenvironment. The relevance of this study lies in its contribution to the knowledge of the obestatin/G protein-coupled receptor system in the tumor context, opening new lines of research on its possible therapeutic applications in pancreatic cancer.
This study investigates the potential therapeutic role of obestatin in the context of pancreatic ductal adenocarcinoma, using an orthotopic mouse model based on the KPC FC1245 cell line. Based on the hypothesis that obestatin could modulate key processes in the tumor microenvironment, the objectives were to analyze its impact on parameters such as apoptosis, autophagy, metastasis, inflammation, hypoxia, senescence, fibrogenesis, and immune response. The methodology employed included the implantation of tumor cells in C57BL/6 mice, treatment with obestatin for fourteen days, and subsequent protein analysis using immunoblot techniques. The main results show that obestatin reduces the expression of senescence markers (p21 and p16) and increases that of a metastasis suppressor (nm23-H1), without significantly altering other parameters such as inflammation or GPR39 receptor expression. These findings indicate a limited but selective effect of obestatin on certain processes associated with tumor progression, suggesting that it could contribute to a less aggressive microenvironment. The relevance of this study lies in its contribution to the knowledge of the obestatin/G protein-coupled receptor system in the tumor context, opening new lines of research on its possible therapeutic applications in pancreatic cancer.
Direction
POMBO RAMOS, CELIA MARIA (Tutorships)
Leal López, Saúl (Co-tutorships)
Pazos Randulfe, Yolanda (Co-tutorships)
POMBO RAMOS, CELIA MARIA (Tutorships)
Leal López, Saúl (Co-tutorships)
Pazos Randulfe, Yolanda (Co-tutorships)
Court
MUÑOZ CREGO, MARIA ANGELES (Chairman)
ROMERO BERNARDEZ, MANUEL (Secretary)
SALGADO CASTRO, FRANCISCO JAVIER (Member)
MUÑOZ CREGO, MARIA ANGELES (Chairman)
ROMERO BERNARDEZ, MANUEL (Secretary)
SALGADO CASTRO, FRANCISCO JAVIER (Member)
Remediation of mine soils in Galicia: use of technosols.
Authorship
E.L.O.
Bachelor of Biology
E.L.O.
Bachelor of Biology
Defense date
09.11.2025 10:00
09.11.2025 10:00
Summary
A bibliographical review has been done on the use of technosols as soil remediation tools in areas damaged by mining activities, with special mention being made of the sulfide-rich mines in Galicia. The impacts produced by this activity are highly serious, as contamination from the accumulation of waste and heavy metals is significant. Contaminated mine soils impact mine water drainage, vegetation growth, and the organisms that inhabit these areas, where bioaccumulation can occur. To minimize, and even eliminate, the possible contamination of these soils, sustainable strategies are needed, such as the use of technosols, artificial soils formed by residues that do not present any danger but that modify and neutralize elements of the soil, thus recovering its essential functions and balance. In addition to Technosols, other soil remediation techniques for degraded soils can be used simultaneously to achieve significant improvements. Amendments and phytotechnologies, combined with technosols, establish synergies that achieve very good results in the restoration of mining soils. In Galicia, there are several examples of mining soils that have been treated with technosols and have almost completely remediated their environmental and productive functions, such as the Touro copper mine and the As Pontes coal mine, both open-pit mines.
A bibliographical review has been done on the use of technosols as soil remediation tools in areas damaged by mining activities, with special mention being made of the sulfide-rich mines in Galicia. The impacts produced by this activity are highly serious, as contamination from the accumulation of waste and heavy metals is significant. Contaminated mine soils impact mine water drainage, vegetation growth, and the organisms that inhabit these areas, where bioaccumulation can occur. To minimize, and even eliminate, the possible contamination of these soils, sustainable strategies are needed, such as the use of technosols, artificial soils formed by residues that do not present any danger but that modify and neutralize elements of the soil, thus recovering its essential functions and balance. In addition to Technosols, other soil remediation techniques for degraded soils can be used simultaneously to achieve significant improvements. Amendments and phytotechnologies, combined with technosols, establish synergies that achieve very good results in the restoration of mining soils. In Galicia, there are several examples of mining soils that have been treated with technosols and have almost completely remediated their environmental and productive functions, such as the Touro copper mine and the As Pontes coal mine, both open-pit mines.
Direction
TABOADA RODRIGUEZ, TERESA MARIA (Tutorships)
TABOADA RODRIGUEZ, TERESA MARIA (Tutorships)
Court
PRIETO LAMAS, BEATRIZ LORETO (Chairman)
BASELGA FRAGA, ANDRES (Secretary)
AMIGO VAZQUEZ, FRANCISCO JAVIER (Member)
PRIETO LAMAS, BEATRIZ LORETO (Chairman)
BASELGA FRAGA, ANDRES (Secretary)
AMIGO VAZQUEZ, FRANCISCO JAVIER (Member)
Biology and conservation of Margaritifera margaritifera: an approach to its situation in Galician rivers
Authorship
P.L.S.
Bachelor of Biology
P.L.S.
Bachelor of Biology
Defense date
09.11.2025 10:00
09.11.2025 10:00
Summary
The freshwater pearl mussel (Margaritifera margaritifera (Linnaeus, 1758)) is a bivalve that plays a key ecological role. Its sensitivity to environmental changes makes it an important bioindicator of water quality and ecosystem health. It stands out for its longevity and high fertility that contrast with a complex life cycle marked by two metamorphoses, key to its survival. This study compiled and synthesized information on the biology and conservation of this mussel, focusing primarily on Galicia, the southern limit of its geographic distribution. This region represents an excellent habitat for this bivalve, which requires clean, cold and oxygenated waters. However, the situation of its populations has recently been strongly conditioned by the alteration of its ecosystem. The increase in eutrophication and temperatures due to human alterations and climate change has caused a decline in populations in recent years, mainly in Europe. This situation made necessary the implementation of conservation measures, among which captive breeding stands out, which is already being carried out in Galicia. Several authors emphasize the need for more studies to deepen knowledge about the characteristics and requirements of this mussel. This would allow improving the effectiveness of conservation measures since there are still gaps in information in different aspects, particularly the limited data available on its ecology.
The freshwater pearl mussel (Margaritifera margaritifera (Linnaeus, 1758)) is a bivalve that plays a key ecological role. Its sensitivity to environmental changes makes it an important bioindicator of water quality and ecosystem health. It stands out for its longevity and high fertility that contrast with a complex life cycle marked by two metamorphoses, key to its survival. This study compiled and synthesized information on the biology and conservation of this mussel, focusing primarily on Galicia, the southern limit of its geographic distribution. This region represents an excellent habitat for this bivalve, which requires clean, cold and oxygenated waters. However, the situation of its populations has recently been strongly conditioned by the alteration of its ecosystem. The increase in eutrophication and temperatures due to human alterations and climate change has caused a decline in populations in recent years, mainly in Europe. This situation made necessary the implementation of conservation measures, among which captive breeding stands out, which is already being carried out in Galicia. Several authors emphasize the need for more studies to deepen knowledge about the characteristics and requirements of this mussel. This would allow improving the effectiveness of conservation measures since there are still gaps in information in different aspects, particularly the limited data available on its ecology.
Direction
IGLESIAS PIÑEIRO, FRANCISCO JAVIER (Tutorships)
IGLESIAS PIÑEIRO, FRANCISCO JAVIER (Tutorships)
Court
PRIETO LAMAS, BEATRIZ LORETO (Chairman)
BASELGA FRAGA, ANDRES (Secretary)
AMIGO VAZQUEZ, FRANCISCO JAVIER (Member)
PRIETO LAMAS, BEATRIZ LORETO (Chairman)
BASELGA FRAGA, ANDRES (Secretary)
AMIGO VAZQUEZ, FRANCISCO JAVIER (Member)
High intensity focused ultrasounds as treatment for Parkinson's disease.
Authorship
T.M.C.
Bachelor of Biology
T.M.C.
Bachelor of Biology
Defense date
09.12.2025 09:00
09.12.2025 09:00
Summary
Parkinson’s disease is a neurodegenerative disease characterized by the progressive loss of dopaminergic neurons and the presence of motor symptoms, such as resting tremor, rigidity, and bradykinesia. Conventional treatment focuses on symptomatic control trough medication, with levodopa being the most relevant drug. However, the progressive loss of its effectiveness and the occurrence of significative side effects have led to the search for alternative therapies, such as deep brain stimulation. Nevertheless, this is an invasive surgical technique that is not suitable for all patients. High intensity focused ultrasounds represents a minimally invasive technique that uses sound waves to ablate specific tissues. This method has previously been successfully used in the treatment of various tumours and neurological disorders, such as essential tremor, which has prompted its consideration for use in patients with Parkinson’s disease. In this study, we aimed to understand the principles of this technique, its applications in neurological diseases, and particularly in Parkinson’s disease. Our results show that clinical trials are being conducted using focused ultrasound in Parkinson’s patients targeting different therapeutic sites. In general, studies report positive outcomes supporting its efficacy and safety. However, there are still some limitations, such as the potential recurrence of symptoms and technical challenges related to targeting certain brain areas. Despite these challenges, technological advances continue to improve its precision and applicability, positioning high-intensity focused ultrasound as a promising therapeutic alternative for patients with Parkinson’s disease.
Parkinson’s disease is a neurodegenerative disease characterized by the progressive loss of dopaminergic neurons and the presence of motor symptoms, such as resting tremor, rigidity, and bradykinesia. Conventional treatment focuses on symptomatic control trough medication, with levodopa being the most relevant drug. However, the progressive loss of its effectiveness and the occurrence of significative side effects have led to the search for alternative therapies, such as deep brain stimulation. Nevertheless, this is an invasive surgical technique that is not suitable for all patients. High intensity focused ultrasounds represents a minimally invasive technique that uses sound waves to ablate specific tissues. This method has previously been successfully used in the treatment of various tumours and neurological disorders, such as essential tremor, which has prompted its consideration for use in patients with Parkinson’s disease. In this study, we aimed to understand the principles of this technique, its applications in neurological diseases, and particularly in Parkinson’s disease. Our results show that clinical trials are being conducted using focused ultrasound in Parkinson’s patients targeting different therapeutic sites. In general, studies report positive outcomes supporting its efficacy and safety. However, there are still some limitations, such as the potential recurrence of symptoms and technical challenges related to targeting certain brain areas. Despite these challenges, technological advances continue to improve its precision and applicability, positioning high-intensity focused ultrasound as a promising therapeutic alternative for patients with Parkinson’s disease.
Direction
RODRIGUEZ PALLARES, JANNETTE (Tutorships)
COSTA BESADA, MARIA ALICIA (Co-tutorships)
RODRIGUEZ PALLARES, JANNETTE (Tutorships)
COSTA BESADA, MARIA ALICIA (Co-tutorships)
Court
MUÑOZ CREGO, MARIA ANGELES (Chairman)
ROMERO BERNARDEZ, MANUEL (Secretary)
SALGADO CASTRO, FRANCISCO JAVIER (Member)
MUÑOZ CREGO, MARIA ANGELES (Chairman)
ROMERO BERNARDEZ, MANUEL (Secretary)
SALGADO CASTRO, FRANCISCO JAVIER (Member)
Development of Alternative Techniques for the Pharmacokinetic Monitoring of Linezolid in Patients with Osteoarticular Infections.
Authorship
M.N.S.
Bachelor of Biology
M.N.S.
Bachelor of Biology
Defense date
09.12.2025 09:00
09.12.2025 09:00
Summary
Linezolid is an oxazolidinone-class antibiotic effective against multidrug-resistant Gram-positive bacteria and commonly used in complex infections, including osteoarticular infections. Due to its high interindividual pharmacokinetic variability and the extended duration of treatment, therapeutic drug monitoring (TDM) is essential for optimizing dosing and minimizing the risk of toxicity or therapeutic failure. In this context, alternative biological matrices, such as oral fluid, and microsampling techniques like volumetric absorptive microsampling (VAMS) offer a less invasive and potentially more accessible alternative to conventional venipuncture. This study forms part of the validation process of a bioanalytical method based on liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for the quantification of linezolid and its main metabolites (PNU-142300 and PNU-142586) in matrices collected through minimally invasive sampling. Specifically, three experimental assessments were carried out to contribute to method development: (1) evaluation of analyte stability in the autosampler; (2) assessment of the potential impact of hematocrit on quantification in dried blood samples obtained using VAMS devices; and (3) verification of the method’s selectivity in saliva and dried blood. For this purpose, spiked samples were collected using VAMS and Salivette devices and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) The results indicate that the analytes remain stable under the tested conditions, the influence of hematocrit is minimal, and no significant interferences were observed in the evaluated matrices. These findings support the feasibility of the method and its potential for future application in clinical settings as a reliable tool for personalized therapeutic monitoring, particularly in scenarios where conventional blood sampling is not feasible.
Linezolid is an oxazolidinone-class antibiotic effective against multidrug-resistant Gram-positive bacteria and commonly used in complex infections, including osteoarticular infections. Due to its high interindividual pharmacokinetic variability and the extended duration of treatment, therapeutic drug monitoring (TDM) is essential for optimizing dosing and minimizing the risk of toxicity or therapeutic failure. In this context, alternative biological matrices, such as oral fluid, and microsampling techniques like volumetric absorptive microsampling (VAMS) offer a less invasive and potentially more accessible alternative to conventional venipuncture. This study forms part of the validation process of a bioanalytical method based on liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for the quantification of linezolid and its main metabolites (PNU-142300 and PNU-142586) in matrices collected through minimally invasive sampling. Specifically, three experimental assessments were carried out to contribute to method development: (1) evaluation of analyte stability in the autosampler; (2) assessment of the potential impact of hematocrit on quantification in dried blood samples obtained using VAMS devices; and (3) verification of the method’s selectivity in saliva and dried blood. For this purpose, spiked samples were collected using VAMS and Salivette devices and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) The results indicate that the analytes remain stable under the tested conditions, the influence of hematocrit is minimal, and no significant interferences were observed in the evaluated matrices. These findings support the feasibility of the method and its potential for future application in clinical settings as a reliable tool for personalized therapeutic monitoring, particularly in scenarios where conventional blood sampling is not feasible.
Direction
Aguiar Fernández, Pablo (Tutorships)
Mondelo García, Cristina (Co-tutorships)
Aguiar Fernández, Pablo (Tutorships)
Mondelo García, Cristina (Co-tutorships)
Court
MUÑOZ CREGO, MARIA ANGELES (Chairman)
ROMERO BERNARDEZ, MANUEL (Secretary)
SALGADO CASTRO, FRANCISCO JAVIER (Member)
MUÑOZ CREGO, MARIA ANGELES (Chairman)
ROMERO BERNARDEZ, MANUEL (Secretary)
SALGADO CASTRO, FRANCISCO JAVIER (Member)
Immunotherapy on solid tumors treatment
Authorship
L.N.S.
Bachelor of Biology
L.N.S.
Bachelor of Biology
Defense date
09.12.2025 09:00
09.12.2025 09:00
Summary
On this review we are going to make an introduction on immunotherapy and the many immunotherapy treatments available to this day. Then we will focus on solid tumors studies, referring to their peculiarities and focus on more detail on non-small-cell lung cancer. We will also make a brief commentary on its combination with radiotherapy treatments, and also about its advantages and limitations. Finally we will assess the topics of discussion on a brief commentary about the future of immunotherapy.
On this review we are going to make an introduction on immunotherapy and the many immunotherapy treatments available to this day. Then we will focus on solid tumors studies, referring to their peculiarities and focus on more detail on non-small-cell lung cancer. We will also make a brief commentary on its combination with radiotherapy treatments, and also about its advantages and limitations. Finally we will assess the topics of discussion on a brief commentary about the future of immunotherapy.
Direction
VIDAL FIGUEROA, ANXO (Tutorships)
TOBIO AGEITOS, ARACELI (Co-tutorships)
VIDAL FIGUEROA, ANXO (Tutorships)
TOBIO AGEITOS, ARACELI (Co-tutorships)
Court
MUÑOZ CREGO, MARIA ANGELES (Chairman)
ROMERO BERNARDEZ, MANUEL (Secretary)
SALGADO CASTRO, FRANCISCO JAVIER (Member)
MUÑOZ CREGO, MARIA ANGELES (Chairman)
ROMERO BERNARDEZ, MANUEL (Secretary)
SALGADO CASTRO, FRANCISCO JAVIER (Member)
Study of New Immunotherapeutic Strategies in the Treatment of Ovarian Cancer
Authorship
J.R.V.
Biotechnology Degree (2nd Ed. )
J.R.V.
Biotechnology Degree (2nd Ed. )
Defense date
09.11.2025 09:30
09.11.2025 09:30
Summary
Ovarian cancer, particularly high-grade serous carcinoma, presents a high mortality rate, often associated with resistance to platinum-based compounds. Interleukin 6 (IL-6), involved in inflammation and immune evasion processes, has been linked to this resistance, making it a potential therapeutic target. This study investigates the role of IL-6 in cell models of cisplatin-resistant ovarian carcinoma and the ability of the monoclonal antibodies siltuximab, which targets IL-6, and tocilizumab, which targets its receptor, to modulate its activity. Through protein quantification analysis, enzyme-linked immunoassay, and gene expression studies, the possible involvement of IL-6 in resistance to platinum derivatives was observed. The results show the ability of siltuximab to block IL-6 released by tumor cells. These findings support the potential of immunotherapies aimed at blocking the IL-6 signaling pathway as strategies against resistant ovarian cancer.
Ovarian cancer, particularly high-grade serous carcinoma, presents a high mortality rate, often associated with resistance to platinum-based compounds. Interleukin 6 (IL-6), involved in inflammation and immune evasion processes, has been linked to this resistance, making it a potential therapeutic target. This study investigates the role of IL-6 in cell models of cisplatin-resistant ovarian carcinoma and the ability of the monoclonal antibodies siltuximab, which targets IL-6, and tocilizumab, which targets its receptor, to modulate its activity. Through protein quantification analysis, enzyme-linked immunoassay, and gene expression studies, the possible involvement of IL-6 in resistance to platinum derivatives was observed. The results show the ability of siltuximab to block IL-6 released by tumor cells. These findings support the potential of immunotherapies aimed at blocking the IL-6 signaling pathway as strategies against resistant ovarian cancer.
Direction
TOBIO AGEITOS, ARACELI (Tutorships)
VIDAL FIGUEROA, ANXO (Co-tutorships)
SARTAGES GARCIA, MIRIAM (Co-tutorships)
TOBIO AGEITOS, ARACELI (Tutorships)
VIDAL FIGUEROA, ANXO (Co-tutorships)
SARTAGES GARCIA, MIRIAM (Co-tutorships)
Court
LEIRO VIDAL, JOSE MANUEL (Chairman)
PARGA MARTIN, JUAN ANDRES (Secretary)
MAURICIO IGLESIAS, MIGUEL (Member)
LEIRO VIDAL, JOSE MANUEL (Chairman)
PARGA MARTIN, JUAN ANDRES (Secretary)
MAURICIO IGLESIAS, MIGUEL (Member)
Analysis of Bioactive Peptides in Cheese Production: A Bibliographic and Bioinformatic Study
Authorship
S.R.P.
Biotechnology Degree (2nd Ed. )
S.R.P.
Biotechnology Degree (2nd Ed. )
Defense date
09.11.2025 09:30
09.11.2025 09:30
Summary
Bioactive peptides are small-sized molecules with significant potential in the food industry. This work explores the technological foundations of cheese-making and how these influence the generation of bioactive peptides derived from milk proteins. Their origin, characterization, and nutritional relevance are analyzed, along with the various biological functions they perform, including antihypertensive, antioxidant, antimicrobial, anticancer, immunomodulatory, and antidiabetic activities. Potential adverse effects are also addressed, such as those associated with β-casomorphin-7. Additionally, the study presents the models used in the identification and evaluation of these peptides, along with the methodological tools employed. Special attention is given to fermentation processes as a means of producing bioactive peptides in cheese, highlighting the technological and microbiological factors involved. Finally, some future perspectives are discussed regarding the use of bioactive peptides of both dairy and non-dairy origin.
Bioactive peptides are small-sized molecules with significant potential in the food industry. This work explores the technological foundations of cheese-making and how these influence the generation of bioactive peptides derived from milk proteins. Their origin, characterization, and nutritional relevance are analyzed, along with the various biological functions they perform, including antihypertensive, antioxidant, antimicrobial, anticancer, immunomodulatory, and antidiabetic activities. Potential adverse effects are also addressed, such as those associated with β-casomorphin-7. Additionally, the study presents the models used in the identification and evaluation of these peptides, along with the methodological tools employed. Special attention is given to fermentation processes as a means of producing bioactive peptides in cheese, highlighting the technological and microbiological factors involved. Finally, some future perspectives are discussed regarding the use of bioactive peptides of both dairy and non-dairy origin.
Direction
CALO MATA, MARIA DEL PILAR (Tutorships)
Barros Velázquez, Jorge (Co-tutorships)
CALO MATA, MARIA DEL PILAR (Tutorships)
Barros Velázquez, Jorge (Co-tutorships)
Court
LEIRO VIDAL, JOSE MANUEL (Chairman)
PARGA MARTIN, JUAN ANDRES (Secretary)
MAURICIO IGLESIAS, MIGUEL (Member)
LEIRO VIDAL, JOSE MANUEL (Chairman)
PARGA MARTIN, JUAN ANDRES (Secretary)
MAURICIO IGLESIAS, MIGUEL (Member)
Influence of the intensity of agricultural practices on micromammal communities in the galician context.
Authorship
S.R.C.
Bachelor of Biology
S.R.C.
Bachelor of Biology
Defense date
09.11.2025 10:00
09.11.2025 10:00
Summary
The galician rural landscape started to shift towards a more intensive agricultural model by the second half of the last century. As a consequence, the complexity of the agropecuarian landscape decreases, the mean size of the patches grows, the total length of hedgegrows shrinks, and monoculture prevails over the mixed landscape mosaic. It was theorized that the intensification of the agricultural model would negatively affect the small mammal biodiversity through habitat modification. To test this hypothesis, this study analized the small mammal communities' composition in eigth sites from the inland Galiza, in a region in which plenty different agricultural models can be found. The sampling consisted on the collection of common barn owl pellets (Tyto alba) in order to identify the preys afterwards. Simultaneously, a characterization of the localities was conducted, considering a series of environmental variables: proportion of each type of land cover, hedgegrow density and landscape heterogeneity. No clear correlation was found between landscape complexity (environmental variables) and the communities' biodiversity and composition. The lack of significance among the results implies the neccesity to further develop ambitious studies that may help comprehend the galician agrosystems' dynamics. It is considered that a local approach that fits the particularities of the study area is necessary, in order to complement the existent information in this field of ecological knowledge.
The galician rural landscape started to shift towards a more intensive agricultural model by the second half of the last century. As a consequence, the complexity of the agropecuarian landscape decreases, the mean size of the patches grows, the total length of hedgegrows shrinks, and monoculture prevails over the mixed landscape mosaic. It was theorized that the intensification of the agricultural model would negatively affect the small mammal biodiversity through habitat modification. To test this hypothesis, this study analized the small mammal communities' composition in eigth sites from the inland Galiza, in a region in which plenty different agricultural models can be found. The sampling consisted on the collection of common barn owl pellets (Tyto alba) in order to identify the preys afterwards. Simultaneously, a characterization of the localities was conducted, considering a series of environmental variables: proportion of each type of land cover, hedgegrow density and landscape heterogeneity. No clear correlation was found between landscape complexity (environmental variables) and the communities' biodiversity and composition. The lack of significance among the results implies the neccesity to further develop ambitious studies that may help comprehend the galician agrosystems' dynamics. It is considered that a local approach that fits the particularities of the study area is necessary, in order to complement the existent information in this field of ecological knowledge.
Direction
DOMINGUEZ CONDE, JESUS (Tutorships)
DOMINGUEZ CONDE, JESUS (Tutorships)
Court
PRIETO LAMAS, BEATRIZ LORETO (Chairman)
BASELGA FRAGA, ANDRES (Secretary)
AMIGO VAZQUEZ, FRANCISCO JAVIER (Member)
PRIETO LAMAS, BEATRIZ LORETO (Chairman)
BASELGA FRAGA, ANDRES (Secretary)
AMIGO VAZQUEZ, FRANCISCO JAVIER (Member)