ECTS credits ECTS credits: 3
ECTS Hours Rules/Memories Student's work ECTS: 51 Hours of tutorials: 3 Expository Class: 9 Interactive Classroom: 12 Total: 75
Use languages Spanish, Galician
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology
Areas: Biochemistry and Molecular Biology
Center Faculty of Medicine and Dentistry
Call:
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
Provide students with a comprehensive review of the underlying concepts, history, issues addressed at present and its projection into the future of molecular biology. We will study the anatomy of the genome and chromatin structure, and analyze the basic processes of DNA replication, repair and recombination, as well as transcription and translation. Analyze the mechanisms of folding, modification, trafficking and degradation of proteins. Review current knowledge of the structure and organization of the human genome from the chromosome to the nucleotide level. Special emphasis will be on the comparison between genomes of different species as a strategy for studying the evolution, structure and function of genes and noncoding sequences of the human genome and model organisms. It will be introduced to the management of experimental tools in molecular biology.
PART 1. DNA: STRUCTURE, REPLICATION AND REPAIR
1. DNA and the nucleus. Deep sequencing. Structure of genes and genomes. Histones. Levels of DNA packaging. Covalent modification of histones and histone subtypes. Current models of chromatin structure. Different kinds of chromatin structure: molecular basis of its establishment. ENCODE Project: techniques for functional genomics.
2. DNA replication. Molecular mechanism of replication. Enzymatic machinery involved in replication. Replication initiation. Regulation of the stability of the replication fork. Chromatin and Replication. Replisomes. Election of replication origins: “dormant” origins. Molecular combing and other techniques to study DNA replication.
3. DNA repair and recombination. DNA lesions. Types of repair. Repair of double-strand breaks by homologous recombination. Homologous recombination: general mechanisms and its applications in the cell. Transposition and conservative sequence specific recombination.
PART 2. GENE EXPRESSION
1. Transcription. Introducing a eukaryotic gene expression. Transcription process and its components. Synthesis of hnRNAs. Maturation, transport and localization of mRNAs. The process of translation and its components. Folding, modification, trafficking and degradation of proteins.
2. Regulation of transcription. Introduction to the regulation of gene expression and control points. Chromatin structure and transcriptional activity. Regulatory elements and transcription factors. Mechanisms of transcriptional regulation. Particular cases of transcriptional control.
3. Post-transcriptional regulation. Pretranslational Control. Eukaryotic translation regulation. The interferon system as an example of integrated regulation of gene expression.
- ALBERTS, B., A. JOHNSON, J. LEWIS, M. RAFF, 2008 THE MOLECULAR BIOLOGY OF THE CELL GARLAND PUBLISHING.
- LEWIN, B. 2008 Genes IX. Oxford University Press
- PAGEL and A. POMIANKOWSKI (Ed.), 2008 Evolutionary genomics and proteomics. Sinauer Associates Inc., Sunderland, MA.
- WEAVER, R. F., 2008 Molecular Biology.McGraw-Hill International Edition
(Relevant scientific papers for the different topics of the matter will also be used)
1. To be able to make judgments about hypotheses, experimental approaches and experiments in the field of Molecular Biology
2. To become familiar with relevant techniques in the fields of Molecular Biology
3. To design experiments in these fields in order to answer relevant questions
4. Understand the fundamentals of research in molecular biology
5. Provide the main strategies for the identification of genome-wide functional motifs
According to the document "Contingency plan or teaching development in the academic year 2021-22" that develops the agreement of the Governing Council of April 30, 2021, the USC contemplates three possible scenarios for the development of teaching during 2021- 2022 academic year, depending on the evolution of the COVID-19 crisis. The teaching methodology to be applied in scenario 1 is indicated below: adapted normality. The adaptations of this scenario to Scenarios 2: Distance and Scenario 3: Closure of facilities are indicated below in the Contingency Plan in the Observations section.
1. Theoretical classes.
2. Seminars. Collective discussion of a selection of scientific articles relevant to the topics of the course proposed by students and teachers, who will present them individually.
Scenario 2) Spacing.
In the event that scenario 2 prevails, the theoretical classes will be taught virtually, with some possible exceptions that will be taught in person. Seminars and tutorials would be taught virtually.
Scenario 3) Closure of facilities
In this scenario, all teaching would be taught virtually.
All virtual teaching will take place through Microsoft's "Teams" software.
For cases of fraudulent performance of exercises or tests, the provisions of the “Regulations for the evaluation of students academic performance and qualifications review” will apply.
1. A final exam of short questions might be done: giving the student the opportunity to demonstrate the conceptual domain acquired (up to 5 points)
2. Presentation of a scientific paper: the understanding of the study and the clarity of exposition will be taken into account (up to 8 points depending on the realization of the final exam.)
3. Active participation in class with questions, comments and opinions will be valued up to 2 points.
In scenario 1, all exams and tests will be face-to-face, while in scenarios 2 and 3 they will be conducted online through the Moodle Quizzes and Microsoft’s “Teams” software.
Lectures: 7 (T), 14 (S), 1 (Tut), 4 (exam)
Homework:21 (T), 28 (S)
TOTAL: 26 (lectures) +49 (homework)= 75
T= theory
S= seminars
Tut= tutorials
Assistance to classes and seminars
The three official languages of the course are Galician, Spanish and English. It is left to the teacher's authority indiscriminate use of either.
Contingency plans
Scenario 2: distancing (with partial restrictions of physical presence). The expository teaching may be carried out totally or partially electronically. In interactive teaching, seminars and practices, physical and telematic presence may be combined, up to a maximum of 50% of the hours of the subject, when distance requires it. Preferably, the tutorials will be online. The final tests will preferably be in person.
Scenario 3: Closure of the facilities (impossibility of teaching face-to-face). Teaching will be completely telematic, with synchronous (MS Teams) or asynchronous (Moodle) mechanisms. The tutorials will be exclusively telematic. The final tests will be exclusively telematic.
Jose Manuel Martinez Costas
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881815734
- jose.martinez.costas [at] usc.es
- Category
- Professor: University Professor
| Monday | |||
|---|---|---|---|
| 10:30-11:30 | Grupo /CLE_01 | Spanish | R.N.S.-Classroom 10 |
| Wednesday | |||
| 09:30-11:30 | Grupo /CLE_01 | Spanish | R.N.S.-Classroom 10 |