ECTS credits ECTS credits: 3
ECTS Hours Rules/Memories Student's work ECTS: 51 Hours of tutorials: 3 Expository Class: 9 Interactive Classroom: 12 Total: 75
Use languages Spanish, Galician, English
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Departments: Chemical Physics
Areas: Chemical Physics
Center Faculty of Chemistry
Call:
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
The general aim of this course is that the students learn the fundamental aspects of electronic spectroscopy, in particular of fluorescence, and photochemistry. Special attention will be paid to the utility of fluorescence to know the molecular behavior in excited electronic states and in the applications of fluorescence in Chemistry, Biology and Medicine. At the end of the course the student should be able:
• To understand the fundamentals of electronic spectroscopy and fluorescence and the molecular features in excited electronic states.
• To know the fluorescence techniques to measure fluorescence.
• To describe the fluorescence quenching mechanisms and their utility.
• To understand the mechanisms of electronic energy transfer and their use in structural studies.
• To know how to use different fluorescence methods to obtain structural and dynamic information about the molecular and supramolecular environment.
• To know the most important types of fluorescence probes and their applications.
• To do fluorescence measurements confidently and correctly.
1. Fundamentals of electronic spectroscopy and fluorescence spectroscopy
Luminiscent phenomena. Radiative and nonradiative processes. Fluorescence excitation and emission spectra. Fluorescence quantum yield. Fluorescence lifetime. Effect of environment on fluorescence.
2. Experimental techniques
Measurement of fluorescence spectra: the spectrofluorometer. Correction of excitation and emission spectra. Measurement of fluorescence lifetimes. Measurement of fluorescence polarization. Ultrafast techniques. Single-molecule fluorescence. Fluorescence Microscopy.
3. Fluorescence quenching
Collisional or dynamic quenching. Stern-Volmer equation. Static quenching. Static and dynamic quenching. Applications to study complex formation and microheterogeneous systems.
4. Excited electronic states and photochemistry
Excited-state complex formation: excimers and exciplexes. Photoinduced electron transfer. Photoinduced proton transfer. Other photochemical reactions.
5. Electronic energy transfer
Electronic energy-transfer mechanisms. Förster Resonance Energy Transfer (FRET). Applications for the measurement of molecular distances and the study of supramolecular associations. Dexter mechanism of energy transfer: photosensitization and photodynamic therapy.
6. Fluorescence probes
Classes of fluorescence probes: intrinsic and extrinsic. Green Fluorescence Protein. Quantum dots. Applications in biomedicine, analyses, environment, and materials studies.
Joseph R. Lakowicz, Principles of Fluorescence Spectroscopy, 3rd Ed. Springer, New York, 2006.
Bernard Valeur, Molecular Fluorescence. Principles and Applications, 2nd Ed. Wiley-VCH, Weinheim, 2012.
Petr Klán and Jacob Wirz, Photochemistry of Organic Compounds: From Concepts to Practice, Wiley, Chichester, 2009.
Paul R. Selvin y Taekjip Ha, Single-Molecule Techniques. A laboratory manual, Cold Spring Harbor Laboratory Press, New York, 2008.
Review and research articles related to the subject.
Basic and general-subject skills:
CG2. To obtain information from the scientific literature using the appropriate channels and to use the above mentioned information to raise and contextualize a research topic.
CG5. To use scientific terminology in English to discuss experimental results in a professional chemical context.
CG6. To apply correctly the new information technologies to solve problems in the professional activity.
CB7. To apply the acquired knowledge and the ability to solve problems in new environments and in a multidisciplinary context related to the area of study.
CB8. To integrate knowledge and to make judgments from an information, taking into account the social responsibilities and ethics linked to the application of this knowledge and judgments.
Transversal skills:
CT1. To elaborate, write and defend in public scientific and technical reports.
CT3. To work with autonomy and efficiency in the daily practise of the research or the professional activity.
CT4. To appreciate the value of quality and continuous improvement, being rigorous and responsible and behaving with professional ethics.
Subject-specific skills:
CE1. To define specialized concepts, principles, theories and facts of the different Chemistry areas.
CE4. To innovate in the synthesis and chemical analysis methods related to the different areas in Chemistry.
CE7. To work with advanced instrumentation for chemical and structural analysis.
LECTURES:
The methodology of the inverted classroom will be used, where the student works the theoretical concepts asynchronously before the class and resolves related exercises and problems in groups during the class with the help of the professor. For this, the contents of the subject are divided in activity sheets and put at the disposal of the students in the virtual classroom. In class, the theoretical concepts studied will be revised solving questionnaires, exercises and related problems. For each topic, the student will deliver one or two problems solved individually.
SEMINARS:
Classes of practical cases that present experimental data and combine various concepts studied in theory. The student participates actively in these classes and delivers his/her individual resolution of the practical case, which is assessed within the continuous evaluation.
TUTORIALS:
These classes will be used for the oral presentation by the students of a research article directly related to the subject and chosen by the student according to their interests. The assessment of this presentation is part of the continuous evaluation.
The passing grade will be obtained for a final grade of 5 out of 10. The final grade, both of first and second opportunity, will be based on the evaluation of the following aspects:
• 40% of the final mark: continuous evaluation based on the following contributions:
• Evaluation of practical cases: 30%
• Oral presentation of a research article: 10%.
Evaluated competences: CG2 CG5 CG6 CB7 CB8 CE1 CE4 CE7 CT1 CT3.
• 60% of the final mark: evaluation of the final exam of the subject with conceptual questions and problems, complementary to the continuous evaluation both in the first and second opportunity and in any of the scenarios. It will be necessary to obtain a minimum mark of 4 out of 10 in the exam to pass the course.
Evaluated competences: CG2 CG5 CB7 CE1 CE4 CE7 CT4.
The assessment of students who repeat the subject will be governed by the same assessment standards as that of students taking the subject for the first time.
PLAGIARISM AND MISUSE OF TECHNOLOGIES IN THE CONDUCT OF TASKS OR TESTS: "For cases of fraudulent execution of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and revision of qualifications will apply."
In-class work time:
Lectures: 12 h
Seminars: 9 h
Tutorials: 2 h
SUBTOTAL: 23 h
Out-of-class work time:
Assignments, solving exercises: 20 h
Study time: 32 h
TOTAL: 75 h
• Attendance to lectures is highly recommended.
• It is important to keep up to date in studying the course material.
• After reading a chapter in any of the recommended books, it is useful to write a summary of the important points.
• Working problems is essential to learning the course contents.
Maria De La Merced Novo Rodriguez
Coordinador/a- Department
- Chemical Physics
- Area
- Chemical Physics
- m.novo [at] usc.es
- Category
- Professor: University Professor
Wajih Al-Soufi
- Department
- Chemical Physics
- Area
- Chemical Physics
- Phone
- 982824114
- wajih.al-soufi [at] usc.es
- Category
- Professor: University Professor
| Friday | |||
|---|---|---|---|
| 12:00-14:00 | Grupo /CLE_01 | English | Classroom 3.44 |
| 01.14.2025 16:00-20:00 | Grupo /CLE_01 | Inorganic Chemistry Classroom (1st floor) |