ECTS credits ECTS credits: 3
ECTS Hours Rules/Memories Hours of tutorials: 3 Expository Class: 10 Interactive Classroom: 17 Total: 30
Use languages Spanish, Galician, English
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Departments: Pharmacology, Pharmacy and Pharmaceutical Technology, External department linked to the degrees, Functional Biology
Areas: Pharmacy and Pharmaceutical Technology, Área externa M.U en Nanociencia e Nanotecnoloxía, Ecology
Center Faculty of Pharmacy
Call: Second Semester
Teaching: Sin Docencia (Ofertada)
Enrolment: No Matriculable (Sólo Alumnado Repetidor)
Due to their nanometric size and their high surface / volume ratio, nanomaterials can have a greater penetration power in organs, tissues and cells than materials with a macrometric size, in some cases causing damage to these structures. The objective of the subject is to give an overview of the potential harmful effects produced by nanomaterials, as well as the different methods of evaluating toxicity in biological, aquatic and environmental systems.
Exhibition class program (10 h)
1. Toxicity: introduction
2. Routes of entry of nanomaterials in the human body
• respiratory, oral, dermal, intradermal, intravenous, intranasal, ocular and other mucosa
• pharmacokinetic profile and bioaccumulation
3. Influence of physicochemical properties on cellular interaction
• hydrophobicity, lipophilicity, catalytic activity, composition, shape
• size, load, surface
• formation of the protein crown in physiological medium
• combination of materials
4. In vitro studies
• toxicity indices and dose-response relationship
• cellular toxicity, ROS, activation of signaling pathways
• genotoxicity and epigenetic toxicity
5. In vivo studies
• animal models
• acute and chronic toxicity
• cardio-hepato-renal-pulmonary toxicity
6. Hemotoxicity
• erythrocytes, platelets, coagulation
• protein interaction, conformational / structure-function changes
7. Immunotoxicity
• cells of the innate and adaptive immune system, cytokines, complement cascade, antibodies
• activation / suppression of the immune system
• techniques to assess immunotoxicity
8. Ecotoxicodynamic
• Biological mechanisms of resistance
• Ecotoxicological effects (population, community and ecosystem levels) of atmospheric and aquatic pollutants. Bioconcentration and biomagnification
9. Risk assessment
• Prediction of ecological risk assessment. Potential toxic activity
• Procedures for ecological risk assessment
Interactive class program (6 h)
1) Seminars and practical blackboard classes: explanation of practical cases and discussion of related publications (UVigo 5 hours, USC 3 hours)
• Discussion of bibliography related to the topics covered in the lectures (techniques, toxicity associated with certain materials, regulation, etc.) and problem solving or exercises provided in class or through the teledoaching platform enabled for the subject.
2) Presentation by one or more students of a work aimed at applying the student's knowledge in the subject (UVigo and USC, 3 hours)
• Oral presentation of an article or review related to Nanotoxicology or Ecotoxicology.
Practical classes program (6 h)
The practices are divided into two sessions of 3 hours each, in which students will perform the following techniques:
• Incubation of various nanomaterials with two cell types, macrophages and lung endothelial cells, and study by flow cytometry of:
-induction of cell death by apoptosis or necrosis by staining with Annexin V / propidium iodide (IP).
-release of reactive oxygen species (ROS) using a specific fluorescent marker for ROS.
• Characterization of possible contaminants present in the materials:
- Bacterial contamination studies on LB agar plates.
- detection and quantification of the presence of endotoxin using the LAL test (Limulus amebocyte lysate)
Bibliografía básica
• Christine Vauthier and Gilles Ponchel. Polymer nanoparticles for nanomedicine. Springer. 2016, ISBN. 978-3-319-41419-5
• Raj Bawa et al. Immune aspects of biopharmaceuticals and nanomedicines. Pan Standford series on Nanomedicine, 2019. ISBN: 978-981-4744-52-9
• Marina A Dobrovolskaia, Scott E McNeil. Handbook of immunological properties of engineered nanomaterials. Volumes 1-3. 2016. ISBN: 9789814699198/ 9789814699204/9789814699211
• Uche Chude-Okonkwo and Reza Malekian. Advanced targeted nanomedicine: a communication engineering solution (Nanomedicine and nanotoxicology).2019. Springer. ISBN-10: 3030110028 / ISBN-13: 978-3030110024
• Nelson Duran, Silvia S Guterres, et al. Nanotoxicology. 2016, Springer ISBN-10: 1493947931/ ISBN-13: 978-1493947935
Bibliografía complementaria
-Literatura científica actual (artículos tipo review y tutorials) proporcionada por el profesorado y centrada en los temas de la asignatura.
Basic:
CB7: That students know how to apply the acquired knowledge and their problem-solving capacity in new or little-known environments within broader (or multidisciplinary) contexts related to their area of study.
CB9: That the students know how to communicate their conclusions –and the ultimate knowledge and reasons that support them– to specialized and non-specialized audiences in a clear and unambiguous way.
CB10: That students possess the learning skills that allow them to continue studying in a way that will have to be largely self-directed or autonomous.
General:
CG1: Master information retrieval techniques related to primary and secondary information sources (including databases with the use of a computer) and critical analysis of information, in Spanish and English.
CG2: Know how to apply knowledge to problem solving in the multidisciplinary field of research and innovation related to nanoscience and nanotechnology.
CG4: Have the capacity to understand the regulation and social responsibilities derived from research, development and innovation in the area of nanoscience and nanotechnology.
CG5: Have knowledge and skills to participate in research projects and scientific or technological collaborations, in interdisciplinary contexts and with a high component of knowledge transfer.
CG7: Be able to safely use nanomaterials in a safe way, respecting current regulations on the prevention of occupational hazards and waste treatment.
CG9: Have oral and written communication skills and scientific interaction with professionals from other areas of knowledge.
CG10: Acquire the necessary training to be able to join future doctoral studies in Nanoscience and Nanotechnology, or in related fields.
Transverse:
CT2: Know how to develop collaborative work in multidisciplinary teams.
CT4: Have the capacity to manage research, development and technological innovation in nanoscience and nanotechnology.
Specific:
CE02 - Interrelate the chemical structure, architecture or arrangement of the nanostructured material with its chemical, physical and biological properties.
CE07 - Know the interactions of nanostructured materials with living things and the environment.
CE08 - Know the main applications of nanomaterials in various fields of knowledge such as physics, chemistry, engineering, biomedicine, biotechnology, or art, among others.
CE10- Understand the design and characterization stages of nanostructured systems for the release of active substances and / or encapsulation / confinement of biomarkers or harmful substances, evaluation of their efficacy and safety.
- Theoretical classes with the participation of the students: exposition by the teacher of the contents of the subject matter under study, theoretical bases and / or guidelines of a work, exercise or project to be developed by the student. Participation through questions that can be posed by the teacher to the students and vice versa.
- Discussion of practical cases in seminars with the support of computer methods and a blackboard: cases / exercises related to what is exposed in the master classes will be proposed.
- Oral presentations / exhibitions: presentation and presentation by the students, individually or in groups, of a topic related to the contents of the subject.
The evaluation will consist of:
- Written exam on the basic contents of the subject (60% of the grade). The examination of the subject, which will be carried out on the date indicated in the corresponding course guide, will consist of test questions, short answers and problem solving. A minimum score of 4 points out of 10 is required in this part for the scores of the other two items that are valued to be computed.
- Active participation in seminars and practical classes (20% of the grade). Active participation in seminars and laboratory practices will be evaluated. This evaluation will be carried out through the resolution of questions and problems posed in class, the presentation of works and the intervention in the debates that may arise.
- Oral presentations (20% of the grade). Expository clarity and the ability to answer the questions that will be asked will be evaluated.
The hours of face-to-face training activities are 30. The hours of personal work of the student are estimated at 45.
The student should avoid the simple memory effort and guide the study to understand, reason and relate the contents of the subject. Participation in interactive activities will allow the student a better understanding of the aspects developed in the expository classes, which will facilitate the preparation of the final exam.
In cases of fraudulent performance of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and review of grades will apply.
Jesus Ramon Aboal Viñas
- Department
- Functional Biology
- Area
- Ecology
- Phone
- 881813311
- jesusramon.aboal [at] usc.es
- Category
- Professor: University Professor