ECTS credits ECTS credits: 3
ECTS Hours Rules/Memories Student's work ECTS: 50 Hours of tutorials: 1 Expository Class: 4 Interactive Classroom: 20 Total: 75
Use languages English
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology, Chemical Physics
Areas: Biochemistry and Molecular Biology, Chemical Physics
Center Faculty of Chemistry
Call: First Semester
Teaching: With teaching
Enrolment: Enrollable | 1st year (Yes)
- Know the different types of microscopy techniques and their theoretical basis.
- Know the different methods and requirements for the preparation of samples for different types of microscopy.
- Get clear notions about the differences between different microscopy techniques and its uses.
- The optical microscope: structure and basic operation. Preparation of samples. Phase contrast. Visualization of living cells.
- Fluorescence microscopy. FRAP and FRET. 3D optical sectioning: deconvolution and confocal microscopy. Visualization of unique molecules by TIRF. Super-resolution microscopy: types. Treatment and preparation of samples for fluorescence microscopy.
- Transmission (TEM) and scanning electron microscope (SEM): operation basics. Preparation of samples for TEM. Contrast and negative staining. 3D reconstruction by tomography. Cryo-microscopy and determination of structures by single particle reconstruction.
- Scanning probe microscopy: bases and types. Preparation of sample. Application to materials and biological samples.
1.- Molecular Biology of the Cell, B. Alberts et all, Garland Science, 2014
Basic
Ð CB6: Possess and understand the knowledge that provides a basis or an opportunity for being creative and unique in the development and/or implementation of ideas, often in a research context.
Ð CB7: Students should know how to use the knowledge acquired and their problem-solving capacity in new or little known environments within wider (or multidisciplinary) contexts related to their field of study.
Ð CB9: Students should know how to communicate their findings and the knowledge and underlying reasons underpinning them to specialised and non-specialised audiences in a clear and unambiguous way.
Ð CB10: Students should have the learning skills that allow them to carry on studying in such a way that should be mainly self-directed or autonomous.
General
Ð CG1: Know how to use the knowledge acquired for practical problem solving in the field of research and innovation, in the multidisciplinary context of biological chemistry and molecular materials.
Ð CG3: Be able to discuss and communicate ideas, in both oral and written form, to specialised and non-specialised audiences (congresses, conferences, etc.) in a clear and reasoned way.
Ð CG4: Be able to understand the social and ethical responsibilities linked to the use of knowledge or judgements in research, development and innovation in the field of biological chemistry and molecular materials.
Ð CG5: Have the skills that allow students to develop an autonomous method for studying and learning.
Ð CG6: Have leadership, creativity, initiative and entrepreneurship abilities.
Ð CG7: Be capable of working in multidisciplinary teams and collaborating with other specialists, both nationally and internationally.
Ð CG8: Be able to use scientific literature and develop the judgement needed for its interpretation and use.
Ð CG10: Be able to develop the different research stages (from the conception of an idea and the literature search through to target setting, experiment design, analysis of the results and drawing conclusions).
Transversal
Ð CT1: Develop teamwork skills: cooperation, leadership and good listening skills. Adapt to multidisciplinary teams.
Ð CT2: Draft scientific and technical reports and defend them publicly.
Ð CT3: Perform day-to-day research or professional activity in an independent and efficient manner.
Ð CT4: Apply the concepts, principles, theories and models related to Biological Chemistry and Molecular Materials to new or little-known environments within multidisciplinary contexts.
Ð CT5: Appreciate the value of good quality and continuous improvement by acting rigorously, responsibly and ethically.
Ð CT6: Be capable of adapting to changes by being self-motivated when applying new and advanced technologies and other relevant developments.
Ð CT7: Show critical and self-critical reasoning when seeking scientific rigour and quality. Handle IT tools and information and communication technology (ICT), as well as on-line access to databases.
Especific
Ð CE4: Know and understand the chemical tools and analytical techniques used for biological chemistry and molecular materials.
Ð CE5: Know how to analyze and use the data obtained autonomously in complex laboratory experiments by relating them to the suitable chemical, physical or biological techniques.
Ð CE7: Students should acquire knowledge on advanced techniques for the structural characterization of macromolecules, supramolecules and colloids which are relevant in the field of biological chemistry and molecular materials.
Ð CE8: Gain technical skill for carrying out the structural characterization of molecules, biomolecules, supramolecules and nanoparticles and interpreting the experimental data obtained.
Ð CE9: Use advanced instrumentation related to research on biological chemistry and molecular materials.
- Interactive classes encouraging student participation.
- Combined use of computer and blackboard methods.
- Work in the laboratory: preparation of samples and management / demonstration of different types of microscopes with prepared samples.
- Promotion of self-learning of the student proposing challenges and posing questions.
- Resolution of practical exercises (problems, questions type test, interpretation and processing of information, evaluation of scientific publications, etc.).
- Oral presentations of previously prepared topics, including discussion with classmates and teachers.
General considerations
- The evaluation process will be used to know if the student has acquired the scheduled skills and to review the teaching methodology.
- Written exam on theoretical and practical basic contents of the subject.
- Continuous evaluation associated with active participation and autonomous learning.
Evaluation weights: Minimum weighting (MiW)- Maximum weight (MaW)
Writen Exam: 50%-70%
Oral presentations: 10%-30%
Other Activities: 20%-40%
Tutoring: 0%-10%
For cases of fraudulent performance of exercises or tests, the provisions of the “Regulations for the evaluation of students academic performance and qualifications review” will apply.
Theoretical classes 3
Seminars 4
Scheduled tutoring 1
Practical laboratory or computer classes 14
Oral exhibitions of the students supported by audiovisual material or lectures by Prof. Guests 2
Evaluation and / or examination 3
SUBTOTAL 27
Non-presential
Preparation of tests and directed works 10
Study and student personal work 35
Bibliographic searches and use of databases 3
SUBTOTAL 48
TOTAL 75 h
Motivation, interaction, initiative and homework, in addition to attend and participate in classes.
Revise basic biochemistry.
Jose Manuel Vila Fungueiriño
- Department
- Chemical Physics
- Area
- Chemical Physics
- josem.vila [at] usc.es
- Category
- Researcher: Juan de la Cierva Programme
Ester Polo Tobajas
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- ester.polo [at] usc.es
- Category
- Researcher: Ramón y Cajal
| Tuesday | |||
|---|---|---|---|
| 17:30-19:00 | Grupo /CLE_01 | English | Mathematics Classroom (3rd floor) |
| Thursday | |||
| 17:30-19:00 | Grupo /CLE_01 | English | Mathematics Classroom (3rd floor) |
| 01.16.2025 16:00-19:00 | Grupo /CLE_01 | Mathematics Classroom (3rd floor) |