Instrumental Analysis

- Introduce students to instrumental analysis as an important part of their training in the field of analytical chemistry and familiarize you with the main electrical, spectrometric and chromatographic analytical techniques.
- Acquiring adequate training in the instrumental analytical methodology.
- Know the fundamentals, characteristics and applications of the main spectroscopic, chromatographic and electroanalytical analytical techniques.
- Addressing real problems inside their future work environment, based on the knowledge acquired.
- Know the calibration and the various aspects of sensitivity and precision of the measurements.
- Know how to apply theoretical principles to advanced troubleshooting and interpret the results obtained from different techniques (spectra, chromatograms, etc.).
- Know the clear selection criteria of the instrumental analysis techniques to be used in every circumstance, so that students can use them in the real world.

THEORETICAL PART:
UNIT 1. INTRODUCTION TO INSTRUMENTAL ANALYSIS
Fundamental concepts. Classification of analytical techniques. Instrumental analysis, classification of instrumental techniques. Analytical instruments. Analytical properties. Quality parameters. Selection of an analytical method. Calibration: how to obtain quantitative information. Most important trends in Instrumental Analysis.
UNIT 2. INTRODUCTION TO THE OPTICAL METHODS OF ANALYSIS.
Introduction. Properties of electromagnetic radiation. Electromagnetic spectrum. Classification of spectroscopic techniques. Absorption Spectroscopy. Beer's law. Optical instruments: Basic components. Terminology.
UNIT 3.- MOLECULAR ABSORPTION SPECTROMETRY. APPLICATION absorption measurements UV-VISIBLE
Introduction. Absorption absorbing species. Tools for absorption. Application of molecular absorption in the UV-VIS: Direct Measurement, derivative spectrophotometry, spectrophotometric titrations.
UNIT 4. PHOTOLUMINESCENCE. AND FLUORESCENCE ANALYSIS
Photoluminescence: fluorescence and phosphorescence. Fluorescence instrumentation. Fluorescence advantages. Fluorescence applications of organic and inorganic components. ¿Photoluminescence or absorption ?.
UNIT 5.- ATOMIC OPTICAL SPECTROMETRY
Introduction. Optical atomic spectra. Optical atomic spectrometry based on flame atomization: Flame atomic techniques – atomic spectra; Flame atomizers; Features of the flames; Atomic absorption spectrometry with flame atomization; Atomic emission spectrometry with flame atomization; Atomic fluorescence spectrometry with flame atomization. Atomic absorption spectrometry with electrothermal atomization (ETAAS). Atomic emission spectrometry with inductively coupled plasma (ICP-AES).
UNIT 6.- INTRODUCTION TO CHROMATOGRAPHIC PROCEDURES.
Overview of chromatography. Migration rates of solutes. Effectiveness of a chromatographic column. Optimizing the operation of a cromatographic column. Chromatography applications.
UNIT 7. LIQUID CHROMATOGRAPHY.
Introduction. Classical liquid chromatography. Instrumentation. Isocratic and gradient. Injectors and detectors. Adsorption chromatography, partition, ion exchange, gel permeation. Applications.
UNIT 8. GAS CHROMATOGRAPHY.
Introduction. Instrumentation. Detection systems. Applications.
UNIT 9. CAPILLARY ELECTROPHORESIS.
Introduction. Theory of capillary electrophoresis. Instrumentation. Capillary electrophoresis methods. Applications.
UNIT 10. INTRODUCTION TO ELECTROCHEMICAL METHODS.
Potentiometry and conductimetría.

SEMINARS:
- Resolution of such problems related to topics taught in the theoretical part.
- Review of selected scientific publications related to the determination of compounds of nutritional interest.

PRACTICES:
- UV-VIS spectrometry.
- Fluorescence Spectrometry.
- Atomic Absorption Spectrometry.
- Atomic Emission Spectrometry.
- High-resolution liquid chromatography.

COURSEWORK:
Students can do group work that will have as themes related to the subject, assumptions, new technology, etc. aspects, which in your case is presented orally.

PRACTICAL FIELD:
Visits to food testing laboratories.
The visits will take place in number, date and time conditioned to the availability of laboratories visited and the existence of the necessary budget for the transfer of the students.

BASIC:
- Principios de análisis instrumental. Skoog, D; Holler, F; Crouch, S.R. Cengage Learning. 2008.
AVAILABLE ON LINE AT: https://www.academia.edu/37326567/Principios_de_an%C3%A1lisis_instrumen…

- Fundamentos de química analítica. Skoog, D .; Holler, J .; Crouch, S.R. ; West, D. M. Thomson, 8th edition, 2005.

OTHER:
- Introducción al Análisis Instrumental. Hernández, L.; González, C. Ed. Ariel Ciencia, 2002.
- Principios de Análisis Instrumental Skoog, D; Holler, F.; Nieman, T. Mcgraw-Hill, 2003.
- Análisis Químico. Métodos y Técnicas Instrumentales Modernas. Teoría Y Ejercicios Resueltos Rouessac, F.; Rouessac, A . Mcgraw-Hill/Interamericana, 2003.
- Análisis Instrumental. Rubinson, K.A.; Rubinson, J.F. Prentice Hall-Pearson Educación, 2000.
- Análisis Instrumental. Skoog, D.; Leary, J. Mcgraw-Hill, 1993.
- Análisis Instrumental. Skoog, D; West, D. Mcgraw-Hill, 1989.
- Métodos Instrumentales de Análisis Willard, H.; Merrit, L.; Dean, J.; Settle, F. Ed. Iberoamericana, 1991.
- Química Analítica Moderna. Harvey, D. Mcgraw-Hill, 2002.
- Química Analítica Contemporánea Rubinson, J.F.; Rubinson, K.A. Pearson Educación, 2000.
- Química Analítica General, Cuantitativa E Instrumental. Bermejo, F. Ed. Paraninfo, 1991.
- Química Analítica Cualitativa. Burriel, F. Ed. Paraninfo, 1989.
- Cálculos de Química Analítica. Hamilton, L.F.; Simpson, S.G.; Ellis, D.W. Ed. Mcgrawhill, 1981.
- Análisis Químico Cuantitativo. Harris, D. Reverté, 2001.
- Estadística y Quimiometría para Química Analítica. Miller, J.N.; Miller, J.C. Prentice Hall, Madrid, 2002.
- Química Analítica. Skoog, D; West, D.; Holler, F.; Crouch, S. Mcgraw-Hill, 2004.
- Fundamentos de Química Analítica. Skoog, D; West, D; Holler, F. Reverté, 1997.
- Principios de Química Analítica. Valcárcel, M. Ed. Springer-Verlag, 1999.

The competences that work in this area are:

Basic competences:
CB1 - That the students have demonstrated knowledge and understanding in a field of study that part of the basis of general secondary education, and is typically a supported by advanced textbooks, includes some aspects that imply knowledge of forefront of their field of study.
CB2 - That students can apply their knowledge to their work or vocation in a professional manner and have competences typically demonstrated through devising and sustaining arguments and solving problems within their field of study.
CB3 - That students have the ability to gather and interpret relevant data (usually within their field of study) to inform judgments that include reflection on relevant social, scientific or ethical.
CB4 - That students can communicate information, ideas, problems and solutions to both specialist and non-specialist audiences.
CB5 - That students have developed those skills needed to undertake further studies with a high degree of autonomy.

Transversal competences:
CT1: Capacity for analysis and synthesis.
CT3: Capacity for teamwork
CT7: Capacity for solving problems.

Specific competences:
COP1 – To know how to use the basic terminology of Instrumental Analysis.
COP2 – To know the basics of the main instrumental analysis techniques, basic components of the instrument, its function and features. And the description and design of the main instrumental configurations

- The exhibition and master classes (35 hours) will be taught by the teachers in charge of the subject, using audiovisual presentations by computer with video projector, with the help of notes on the blackboard.
- The interactive seminars (4 hours) consist of complementary activities subject matter of the lectures, which will be taught by teachers and / or students being supervised by them.
- Practical classes (12 hours) consist of laboratory practices that include a brief theoretical introduction and practice outlines detailing procedures to be developed. Students will work in groups of 2 students. At the end the students will deliver a written report with the results. Attendance at practices is mandatory to pass the subject.
- Field practices will be visits to food testing laboratories (subject to availability of funding).
- Students can do group work related to the topics of the subject, to be presented orally in the classroom. They will also work consisting of individual problem solving numerical assumptions.
- The orientation of all activities and tasks performed by students will be made by teachers in group tutoring sessions (two hours).
- The Virtual Campus of the USC will be used as teaching support.

Student assessment will be done through continuous assessment, conducting work and practice report as well as the final exam.
Valuation percentages for the final evaluation of the student:
Final exam: 65% (Competences assessed: CT1, CT7, COP1, COP2)
Continuous assessment and coursework: 20% (Competences assessed: CB1, CB2, CB3, CB4, CB5, CT1, CT3, CT7, COP1 and COP2).
Practice report: 15%. (Competences assessed: CT3, CT7, COP1, COP2).
Only students who have not made any assessment activities may obtain a final rating of Withdraw.
Failure to pass the subject at the earliest opportunity, the student will be assessed in the Second Chance through a final review of theoretical and practical content.

The subject consists of 6 ECTS credits. The total number of hours the student is 156 hours, of which 36% are face and 64% are student work hours. The detailed time distribution independent student work is as follows:
- Master classes: 35 contact hours / 70 h autonomous work
- Seminars: 4 contact hours / 8 h autonomous work
- Practices: 12 hours classroom / 4 h autonomous work for lab notebook
- Individual work: 2 contact hours / 2 h autonomous work
- Group work: 3h self-employment.
- Tutorials: 2 contact hours / 2 h autonomous work
- Examinations and review: 2 contact hours / 2 h autonomous work

Attending lectures and resolution of doubts in the tutorials is recommended.
Pay attention in lectures, seminars and practices. Take the matter to date.
Study a reasoned and linking the contents of the various topics.

Teaching languages: Galician and Spanish.