ECTS credits ECTS credits: 6
ECTS Hours Rules/Memories Hours of tutorials: 3 Expository Class: 27 Interactive Classroom: 21 Total: 51
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Functional Biology
Areas: Plant Physiology
Center Faculty of Biology
Call: First Semester
Teaching: With teaching
Enrolment: Enrollable
The aim of the subject is for students to acquire the following skills:
- Detail some applications and the current state of the different fields of plant biotechnology.
- List and know how to apply different techniques of propagation and preservation by in vitro culture, including cell cultures.
- Identify the different processes that give rise to a phenotype caused by a genetic manipulation, from the role of the promoters to the alteration of metabolic flows.
- Know how to make different types of genetic constructions for overexpression, silencing and genome editing.
- List and know how to apply different techniques of stable and transient transformation by means of Agrobacterium or biolistic transformation, including the transformation of chloroplasts.
LECTURES:
1. Reproduction: reproduction systems, crossings and polyploidy, 3h
2. Applications in plant breeding: pure lines, hybrids, markers and mapping, 3h
3. In vitro culture: micropropagation, organogenesis, embryogenesis and cell cultures, 2h
4. Functional genomics: Identification of candidate genes and functional characterization, 4h
5. Genetic manipulation: Design of strategies from gene to phenotype, 5h
6. Construction design: Overexpression, silencing and gene editing, 4h
7. Transformation and selection methods, 4h
LABORATORY:
The students will work 14 hours in the laboratory in which the following activities will be carried out:
1. In vitro culture
2. Transitory transformation in Nicotiana benthamiana.
3. Stable transformation in Arabidopsis thaliana.
4. Genetic mapping.
5. Planning of experiments and analysis of results.
SEMINARS:
Preparation of a research project.
TUTORING:
Guidance to students in the preparation of lab reports and presentations.
BASIC
Stewart N. ed., 2012. Plant biotechnology and genetics: principles, techniques, and applications. 2nd ed. Hoboken: John Wiley & Sons Inc.
Altman A., Hasegawa P. M. eds., 2012. Plant biotechnology and agriculture: prospects for the 21st century. Amsterdam: Academic Press.
Plant Biotechnology Journal. https://onlinelibrary.wiley.com/journal/14677652
ADDITIONAL
Slater A., Scott N. W., Fowler M. R. eds., 2008. Plant biotechnology: the genetic manipulation of plants. Oxford: Oxford University Press.
In this subject the student must achieve the following learning outcomes:
KNOWLEDGE/CONTENTS: CON01, CON05
SKILLS/ABILITIES: H/D01, H/D03, H/D05, H/D06, H/D07, H/D10, H/D12, H/D13
COMPETENCES: COMP02, COMP03, COMP04, COMP05, COMP08
The lectures will be taught in 25 one-hour sessions, with a frequency of 3 hours per week. Problem-based learning will be used in them. Students will be provided with notes and problems will be posed, explaining the theoretical aspects necessary to solve them, as well as other doubts that may arise.
Knowledge: Con01, Con05.
Skills: H/D01, H/D05, H/D07.
Competences: Comp03, Comp04, Comp05, Comp08.
The interactive seminar classes will be taught in small groups for 10 sessions. Project-based learning will be used in these classes. Students must develop and defend a research project to solve a problem using the techniques studied in the subject.
Knowledge: Con01, Con05.
Skills: H/D01, H/D03, H/D05, H/D07.
Competencies: Comp02, Comp03, Comp04, Comp05.
The in-person laboratory practices will take place in 14 hours distributed over 5 sessions. Attendance will be checked through participation lists. The students must prepare an individual lab report.
Knowledge: Con01, Con05.
Skills: H/D03, H/D05, H/D06, H/D07, H/D10, H/D12, H/D13.
Competencies: Comp02, Comp03, Comp04, Comp05.
Throughout the course, 2 tutorial sessions will be held in person or virtually in very small groups in which doubts will be resolved and students will be guided in carrying out the gradable assignments.
MANDATORY ACTIVITIES THAT ARE NOT GRADED:
It will be necessary to attend at least 4 practice sessions to be able to present the laboratory report.
MANDATORY GRADED ACTIVITIES:
- Laboratory report (25% of the final grade).
It will be necessary to submit a lab report and obtain a minimum grade of 4 out of 10 to be able to take the final exam. Students who obtain less than 4 may take the second-chance exam if they complete a new lab report that achieves that grade. If you obtain at least a 5 and fail the subject, it will not be necessary to repeat the lab classes and the grade of the report will be maintained in the following two years. Repeating students who obtain at least a 5 may voluntarily submit a new lab report. To do so, it will be necessary to attend at least the two computer lab sessions. A student who does not submit the lab report will not have a grade for the subject.
Knowledge: Con01, Con05.
Skills: H/D03, H/D05, H/D06, H/D07, H/D10, H/D12, H/D13.
Competencies: Comp02, Comp03, Comp04, Comp05.
- Preparation, presentation and discussion of projects (25% of the final grade).
A minimum grade of 4 out of 10 will be required to be eligible to sit the final exam. If you get at least a 5 and fail the course, it will not be necessary to repeat the seminars and the grade will be maintained in the following two years.
Knowledge: Con01, Con05.
Skills: H/D01, H/D03, H/D05, H/D07.
Competencies: Comp02, Comp03, Comp04, Comp05.
- Final exam (50% of the final grade).
The final exam will consist of a study of plant biotechnology cases. During the final exam, any type of printed material may be consulted.
Knowledge: Con01, Con05.
Skills: H/D01, H/D07.
Competencies: Comp03, Comp04, Comp05, Comp08.
FACE-TO-FACE WORK IN THE CLASSROOM:
- Lectures: 25 hours
- Interactive seminar classes: 10 hours
- Practical laboratory classes: 14 hours
- Small group tutorials: 2 hours
- Final exam: 3 hours
STUDENT PERSONAL WORK:
- Individual study and completion of the practical report and research project: 96 hours
TOTAL WORK HOURS: 150 HOURS
- It is advisable to study the subject during the whole course.
- It is important to organize the class notes so that they can be consulted efficiently during the seminars and exams.
The subject material and all announcements will be available in the virtual classroom. A Teams group will be used to share the results of the laboratory classes.
Javier Sampedro Jiménez
Coordinador/a- Department
- Functional Biology
- Area
- Plant Physiology
- Phone
- 881813113
- javier.sampedro [at] usc.es
- Category
- Professor: Temporary PhD professor
Oscar Martinez Troncoso
- Department
- Functional Biology
- Area
- Plant Physiology
- oscar.martinez [at] usc.es
- Category
- Professor: Intern Assistant LOSU
| Monday | |||
|---|---|---|---|
| 10:00-11:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |
| Tuesday | |||
| 09:00-10:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |
| Wednesday | |||
| 12:00-13:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |
| 01.09.2025 10:00-14:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |
| 06.20.2025 10:00-14:00 | Grupo /CLE_01 | Classroom 03. Carl Linnaeus |