ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Student's work ECTS: 74.25 Hours of tutorials: 2.25 Expository Class: 18 Interactive Classroom: 18 Total: 112.5
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology, Microbiology and Parasitology
Areas: Biochemistry and Molecular Biology, Microbiology
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
The aim of this course is for students to acquire a knowledge and understanding of:
1) The development of vaccines through the use of biotechnological tools.
2) The fundamentals and methods for the use of viruses for preventive and therapeutic purposes.
Lectures (20 h):
1. Viral vectors in gene therapy. Design, production and applications. 2 h
2. Viral vectors for recombinant protein expression. Types and applications. 2h
3. Viruses and nanotechnology. Virus like particles (VLPs), viral nanoparticles (VNP), nanoconjugates. Methods and applications 2h.
4. Oncolytic viruses. Viral types and applications. 2h
5. Biotechnological applications of bacteriophages. 1h
6. Baculoviruses as biopesticides. Formulation and applications. 1h
7. Vaccines: definition, characteristics, and composition. History of vaccination. 2h
8. Classification of vaccines. Characteristics, benefits, problems of the different types of vaccines. 2h
9. Vaccination guidelines and routes of administration. Adjuvants. Definition and mechanisms of action. Compounds of traditional use and new research. 1h
10. Design. Identification of response-inducing antigens. New generation vaccines. 2h
11. Development of procedures for the industrial production of vaccines. Vaccine control: legal and sanitary aspects. Requirements for vaccine registration. 1h
12. Vaccination: current challenges and strategies. 2h
Seminars (5 h)
Development of practical exercises and problem solving related to theory topics (activity carried out in the Virtual Campus).
Presentation of assignments and discussion on the exercises performed and specific aspects of the subject.
Practical classes (12 h)
The practical classes will be held in 5 sessions.
1. Cultivation, identification and quantification of viruses 2h
2. Manipulation of viral genomes and transfection 2h
3. Recovery of recombinant viruses and detection of recombinant protein: 2h
4. Basis and expression of a subunit vaccine in bacteria 3h
5. Purification and characterization of subunit vaccine 3h
Tutorials
Resolution of doubts 1h
Basic bibliography
- Bloom, B. R. and Lambert, P. H. (2016) The Vaccine Book: Second Edition, The Vaccine Book: Second Edition. Amsterdam: Elsevier Inc. doi: 10.1016/C2014-0-00804-0.
- Flint, J., Rall, G. F., Racaniello, V. R., & Skalka, A. M. (2020) Principies of Virology. Hoboken: Wiley. Available at: https://www.wiley.com/en-us/Principles+of+Virology%2C+Multi+Volume%2C+5….
- Khan, M. S., Khan, I. A. (Iqrar A. and Barh, D. (2019) Applied molecular biotechnology : the next generation of genetic engineering. Boca Raton: CRC Press. Available at: https://www.routledge.com/Applied-Molecular-Biotechnology-The-Next-Gene….
- Tennant, P., Fermin, G. and Foster, J. E. (Jerome E. (2018) Viruses : molecular biology, host interactions, and applications to biotechnology. London: Academic Press.
Complementary bibliography
- Cann, A. J. (2015) Principles of Molecular Virology: Sixth Edition, Principles of Molecular Virology: Sixth Edition. Amsterdam: Academic Press. doi: 10.1016/C2014-0-01081-7.
Basics
Those specified in the degree report.
General
Those specified in the degree report.
Transversal
CT1 - Think in an integrated way and approach problems from different perspectives.
CT2 - Search, process, analyze and synthesize information from different sources.
CT3 - Organize and plan their work.
CT4 - Interpret experimental results and identify consistent and inconsistent elements.
CT5 -To work in a team.
CT6- To reason critically.
Specific
CE1 - To know how to make calculations, analyze data and interpret experimental results specific to the fields of Biotechnology.
SC3 - Know and know how to apply the instrumental techniques and work protocols in a laboratory, applying the regulations and techniques related to safety and hygiene, waste management and quality.
SC5 - Knowing how to obtain and interpret information from the main databases (biological, omics, bibliographic) and use bioinformatics tools.
CE9 - To know how to identify the different types of viruses, microorganisms and animal and plant tissues, to understand their development, organization and physiology, and to know their application in the field of biotechnology.
CE11 - To know the molecular bases and techniques of manipulation of gene information in microorganisms, animals and plants and to know how to apply these techniques adequately in the different biotechnological fields.
The teaching methodology will be based on lectures, providing concepts, diagrams and photographs through audiovisual media and will encourage active student participation through the formulation of questions or the posing of practical cases to be solved in the classroom. The development of practical exercises and problem solving will be promoted (online activity), which will be corrected during the seminars, in which the work carried out by the students will also be presented (activity aimed at promoting the use of bibliography, interpretation and synthesis of information).
Practical classes will be held in the laboratory.
1-hour tutorials will be scheduled for the resolution of doubts.
1. Compulsory activities
1.1 Demonstration of the acquisition of the concepts of the subject.
Theoretical examination* The student's theoretical knowledge will be evaluated by definition of basic concepts, answers to short questions, test questions and interpretation of images and diagrams. In addition to the contents, clarity and conciseness will be taken into account: 65% (maximum grade 6.5).
[CG3, GG4, CT1, CT2, CE1, CE9, CE11].
1.2. Performance of the practices**
- Evaluation of the practical report (its presentation, the quality of the report and the description and explanation of the tests carried out will be taken into account): 10% (maximum grade 1)
[CT4, CE1, CE3 are evaluated].
2. Continuous evaluation (optional activities)
Completion of assignments or case studies/problems**: 25%.
- Seminar tasks (the quality of the report and the presentation will be taken into account, as well as the confidence in the answer to the questions posed): 15% (maximum grade 1.5)
- Resolution of exercises, problems and practical cases (their presentation and the reasoning in the resolution will be taken into account): 10% (maximum grade 1).
[CB3, CB4, CG3, CG4, CT1, CT2, CT3, CT5, CT6, CE5, CE9, CE11].
* The theoretical exam will be in writing, according to the date established by the decanal team. Optionally, students who so wish may take the oral exam, on a date previously agreed with the interested party(s). The grade of the theoretical exam can be kept only until the 2nd opportunity exam of the current academic year. It will be necessary to pass the theoretical exam (5) in order to add the rest of the items.
** The grade for the practicals and the continuous evaluation activities will be kept for two academic years if the student requests it.
Course of 4.5 ECTS credits x 25 hours = 112.5 hours
Total classroom hours: 40.5
- Lectures: 20 hours
- Practical classes: 12 hours
- Seminars: 5 hours
- Group tutorials: 1
- Individual tutoring: 0.5
- Exam: 2 hours
Personal work: 72 hours
Knowledge of biochemistry, genetics and microbiology is required to take the course.
The subject will have a virtual classroom on the Moodle platform in which, in addition to the contents developed by the teachers, other useful information will be available to students
For cases of fraudulent performance of exercises or tests, the provisions of the "Normativa de avaliación do rendemento académico dos estudantes e de revisión de cualificacións" (Regulations for the assessment of students' academic performance and review of qualifications) shall apply.
Jose Manuel Martinez Costas
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881815734
- jose.martinez.costas [at] usc.es
- Category
- Professor: University Professor
Sandra Souto Pereira
Coordinador/a- Department
- Microbiology and Parasitology
- Area
- Microbiology
- sandra.souto [at] usc.es
- Category
- Xunta Post-doctoral Contract
Daniel Abella López
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- daniel.abella [at] rai.usc.es
- Category
- Ministry Pre-doctoral Contract
Adrián López Teijeiro
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- adrianlopez.teijeiro [at] usc.es
- Category
- Ministry Pre-doctoral Contract
Paula Sanchez Gascon
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- paulasanchez.gascon [at] usc.es
- Category
- USC Pre-doctoral Contract
| Monday | |||
|---|---|---|---|
| 10:00-11:00 | Grupo /CLE_01 | Spanish | Classroom 09. Barbara McClintock |
| Tuesday | |||
| 10:00-11:00 | Grupo /CLE_01 | Spanish | Classroom 09. Barbara McClintock |
| 05.31.2024 10:00-14:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |
| 07.08.2024 10:00-14:00 | Grupo /CLE_01 | Classroom 08. Louis Pasteur |