ECTS credits ECTS credits: 3
ECTS Hours Rules/Memories Student's work ECTS: 51 Hours of tutorials: 3 Expository Class: 9 Interactive Classroom: 12 Total: 75
Use languages Spanish, Galician
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Departments: Physiology
Areas: Physiology
Center Faculty of Medicine and Dentistry
Call: First Semester
Teaching: With teaching
Enrolment: Enrollable | 1st year (Yes)
- To provide the knowledge about the design of the distinct types of vectors used in the generation of modified genetically animals (GMA).
- To provide the knowledge about the main techniques of gene transfer.
- To provide the knowledge and skills for handling the basic bibliography available
- Acquire the skills to peruse the pertinent literature and intelligently and creatively apply it to plan working hypothesis, experimental proposals or experiments already performed, discussing both the scientific validity and the ethical and social aspects.
1. Introduction to the utilization of genetically modified animals (GMAs). Types of GMAs.
2. Specific ethical considerations for the utilization of GMAs.
3. Genetically modified mice (GMM). Generation of genetically modified mice by pronuclear microinjection of DNA. Other methods of generation of genetically modified mice (viral infection, embryonic stem cells (ES) cells). Knock-out mice. Generation of knock-out mice by aggregation of ES cells. Conditional models. Other types of GMM. Knock-in mice. Utilization of the technology of RNAi in the generation of GMM: knock-down mice.
4. Isolation of the gene of interest. Vector design using bioinformatic tools. Cloning of the DNA sequences. Construction of the DNA vectors. Design and construction of the targeting vector used for generating the genetically modified animals (knock-out, knock-in and conditional models). Generation of GMM. Purification of high quality DNA for pronuclear microinjection of DNA and gene targeting by homologous recombination in ES cells. Genotyping: Southern Blot and PCR.
5. Maintenance of colonies of GMM. Facilities, equipment, care and handling. Specific sanitation standards. Use of GMM and experimental design.
6. Phenotyping. Prenatal and postnatal influences. Effect of the genetic background on the phenotype. Phenotyping strategies and examples.
Basic references:
Kaufman,M.H. & Bard,J.B.L. The Anatomical Basic of Mouse Development: Academic Press; 1999.
Papaioannou VE, Behringer RR. Mouse Phenotypes. A handbook of mutation analysis: CSHL Press; 2005.
Popesko P, Rajtova V, Horák J. A colour Atlas of anatomia of small laboratory animals. Vol. 2: Ed. Saunders; 2002.
Holland, EC. Mouse Models of Human Cancer. 1st Ed: Wiley-VCH Verlag GmbH & Co. KGaA; 2004.
Behringer R, Gertsenstein M , Nagy K, Nagy A. Manipulating the Mouse Embryo: A Laboratory Manual, 4th ed: CSHL Press; 2014.
Complementary references:
Nature Biotechnology
Trends in Biotechnology
Current Opinion in Biotechnology
Transgenic Research
Animal Reproduction Science
Links:
http://www.ncbi.nlm.nih.gov/pubmed/
http://emice.nci.nih.gov/emice/
http://www.eumorphia.org
http://www.bugalicia.org
http://busc.usc.es
https://secal.es/wp-content/uploads/2014/10/00-GENETICA-indice.pdf.pdf
Alternative support material:
The support material provided by the professors will be avaliable at CampusVirtual platform:
http://www.usc.es/campusvirtual/
General skills:
General skills:
* Instrumental skills
Use of the necessary tools to carry out proper research of information: electronic resources and periodic publications.
Design effective resolution strategies.
Accurate use of scientific terminology.
Presentation skills and public speaking in Science.
* Systemic skills
Development of critical thinking in Science.
Introduction of the concept of "mutability of knowledge"in Science
Development of the scientific spirit.
* Interpersonal skills.
Development of skills related with the interaction and the work in group.
Specific skills
- Knowledge of the main techniques of gene transfer.
- Design of different types of vectors.
- Knowledge of the practical applications of these technical
- Knowledge of basic bibliography.
- Read, analyse, synthesize and comment scientific papers related with the genetic modification.
Basic skills
In accordance with the established in the R.D. 1393/2007 (point 3.4 of the Annex I) will guarantee the following basic skills:
- That the students have showed a compresión systematic of a field of study and the dominance of the skills and methods of research related with the field.
- That the students have showed the capacity to conceive, design, put in practice and adopt a substantial process of research with academic seriousness.
- That the students have realized a contribution through an original investigation that enlarge the borders of the knowledge developing a substantial corpus, of the that splits deserve the publication referred to national and international level.
- That the students are able to realize a critical analysis, evaluation and synthesis of new and complex ideas.
- That the students know to communicated with the his colleagues, with the academic community and with the society in general in regard to the areas of knowledge.
- That suppose them able to promote, in academic and professional contexts, the technological advance, social and cultural inside a society based in the knowledge.
Theoretical classes:
They constitute the main methodology for knowledge acquisition. They follow the classical model of master class.
Seminars:
They cover aspects directly related with the subject, but that do not form part of the its central body. This format favors the use of research information tools and work in group. The students will have to collect information concerning the subjects of the seminar proposed, realize a public presentation and answer to the questions raised by students and professors.
In the Teaching Methodology, both synchronous and asynchronous telematic means will be used in order to contemplate the possibility of implementing the three possible scenarios: adapted normality, distancing and closure of the facilities.
-Assistance to at least 70% of the sessions; Mandatory condition.
-Participation in class and seminars; oral communication, review of the information; Presentation of the work; 60%
-Experimental design; Presentation of the written work; 40%
In the Assessment System, both synchronous and asynchronous telematic means will be used in order to contemplate the possibility of implementing the three possible scenarios: adapted normality, distancing and closure of the facilities.
Activity Master classes/Seminars Individual tasks Total
Theory 7 21 28
Seminars 14 28 42
Tutorials 1 - 1
Exam and revision 4 - 4
Total 26 49 75
Previous knowledge in cellular and molecular biology.
Victor Manuel Arce Vazquez
- Department
- Physiology
- Area
- Physiology
- Phone
- 881812291
- victor.arce [at] usc.es
- Category
- Professor: University Professor
Francisco Javier Martin Cora
- Department
- Physiology
- Area
- Physiology
- Phone
- 881812295
- franciscoj.martin.cora [at] usc.es
- Category
- Professor: Temporary PhD professor
Jose Antonio Costoya Puente
Coordinador/a- Department
- Physiology
- Area
- Physiology
- Phone
- 881815449
- josea.costoya [at] usc.es
- Category
- Professor: University Lecturer
Miguel Antonio Lopez Perez
- Department
- Physiology
- Area
- Physiology
- Phone
- 881815420
- m.lopez [at] usc.es
- Category
- Professor: University Professor
| Tuesday | |||
|---|---|---|---|
| 10:30-11:30 | Grupo /CLE_01 | Spanish, Galician | R.N.S.-Classroom 10 |
| Thursday | |||
| 10:30-12:30 | Grupo /CLE_01 | Galician, Spanish | R.N.S.-Classroom 10 |