ECTS credits ECTS credits: 3
ECTS Hours Rules/Memories Hours of tutorials: 4 Expository Class: 12 Interactive Classroom: 14 Total: 30
Use languages Spanish, Galician, English
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Departments: Physiology, Pharmacology, Pharmacy and Pharmaceutical Technology, External department linked to the degrees
Areas: Physiology, Pharmacy and Pharmaceutical Technology, Área externa M.U en Nanociencia e Nanotecnoloxía
Center Faculty of Pharmacy
Call: First Semester
Teaching: Sin Docencia (Ofertada)
Enrolment: No Matriculable (Sólo Alumnado Repetidor)
- To understand the importance of the routes of administration, biodistribution, and excretion of nanostructured materials
- To know the active response mechanisms of complex organisms against foreign materials.
- To obtain a comprehensive view of the organism-nanostructured material interactions.
Unit 1. Introduction to Bionanotechnology. Definition of bionanotechnology, areas of science involved, areas of application.
Unit 2. ADME. Administration routes. Adsorption: Penetration into the cell, cell-to-cell transfer, translocation. Distribution: blood and lymphatic circulation, fenestrated vessels (solid tumors). Metabolization. Elimination: Biodegradable; not biodegradable; digestive, urinary, respiratory routes.
Seminar 1. Genetic characterization models (alteration pathways, genes)
Seminar 2. In vitro diagnostic techniques: Dot Blot and Elisa
Seminar 3. Diagnostic imaging techniques
Unit 3. Organism response to nanomaterials. Immune system. Cells and humoral factors. Recognition of the immune system to nanomaterials: Receptors and internalization pathways. Consequences of SI Activation. Consequences of SI Inhibition. Immune responses involved: Phagocytosis; Chemotaxis; Cell activation; Production of reactive oxygen species; Complement activation; Cytokine production; Antibody production; Hypersensitivity responses. Infusional reactions; Basophil activation; Alteration in cell migration; Cytotoxicity; Tolerance induction. Design of techniques to study SI-nanomaterials interaction
Seminar 4. Cell analysis techniques: Flow cytometry, microscopy
Topic 4. Therapeutic applications
Seminar 5. HTS
Seminar 6. Animal models
Seminar 7. Adverse effects
LABORATORY PRACTICES
1. DotBlot
2. ELISA
3. Lateral immunochromatography with gold nanos
4. Phagocytosis
- Medical Pharmacology at a Glance. Michael J. Neal. John Wiley & Sons Inc; Edición 8th, 2016.
- Fisiología Médica - 3ª edición, Walter F. Boron, Emile L. Boulpaep. Elsevier, 2017.
- The Handbook of Nanomedicine, Kewal K. Jain. Humana Press, 2012.
- Tratado General de Biofarmacia y Farmacocinética. Vol. I y II. Domenech J., Martínez J. y Peraire C. Síntesis. Madrid 2013.
Basic:
CB6: Possess and understand knowledge that provides a basis or opportunity to be original in the development and / or application of ideas, often in a research context.
CB7: That students know how to apply the acquired knowledge and their problem-solving capacity in new or little-known environments within broader (or multidisciplinary) contexts related to their area of study;
CB8: That students are able to integrate knowledge and face the complexity of formulating judgments based on information that, being incomplete or limited, includes reflections on the social and ethical responsibilities linked to the application of their knowledge and judgments;
CB9: That students know how to communicate their conclusions –and the ultimate knowledge and reasons that support them– to specialized and non-specialized audiences in a clear and unambiguous way;
CB10: That students possess the learning skills that allow them to continue studying in a way that will have to be largely self-directed or autonomous.
General:
CG2: Know how to apply knowledge to problem solving in the multidisciplinary field of research and innovation related to nanoscience and nanotechnology.
CG4: Have the capacity to understand the regulation and social responsibilities derived from research, development and innovation in the area of nanoscience and nanotechnology.
CG5: Have knowledge and skills to participate in research projects and scientific or technological collaborations, in interdisciplinary contexts and with a high component of knowledge transfer.
CG6: Have leadership capacity, creativity, initiative and entrepreneurial spirit.
CG8: Know how to apply the knowledge and skills acquired for planning and integrated project management.
CG9: Have oral and written communication skills and scientific interaction with professionals from other areas of knowledge.
Transverse:
CT1: Know how to propose a simple research project autonomously in Spanish and English.
CT2: Know how to develop collaborative work in multidisciplinary teams.
CT3: Use Information and Communication Technologies (ICTs) as a tool for the transmission of knowledge, results and conclusions in specialized fields in a clear and rigorous way.
CT4: Have the capacity to manage research, development and technological innovation in nanoscience and nanotechnology.
CT5: Know how to apply the principles contained in The European Charter & Code for Researchers.
Specific:
CE07 - Know the interactions of nanostructured materials with living things and the environment.
- Theoretical classes with student participation.
- Discussion of practical cases in seminars with the support of computer methods and a blackboard.
- Problem-based learning
- Oral presentations of previously prepared topics, followed by debate with the participation of students and teachers.
- Attendance at conferences or round tables
- Laboratory practices. Practices will take place at the University of Vigo and students must travel by their own means.
The evaluation will consist of:
- Written exam on the basic contents of the subject (50% of the grade). The exam of the subject, which will be held on the date indicated in the corresponding course guide, will consist of short answer questions and problem solving. The maximum score will be 5 points. A minimum score of 2 points is required in this part for the scores of the other two items that are valued to be computed.
- Active participation in seminars and practical classes (30% of the grade). Active participation in seminars and laboratory practices will be evaluated. This evaluation will be carried out through the resolution of questions and problems raised in class, the presentation of works and the intervention in the debates that may arise. The maximum score will be 3 points.
- Oral presentations (20% of the grade). Expository clarity and the ability to answer the questions that will be asked will be evaluated. The maximum score will be 2 points.
The hours of face-to-face training activities are 30. The hours of personal work of the student are estimated at 45.
The student should avoid the simple memory effort and guide the study to understand, reason and relate the contents of the subject. Participation in interactive activities will allow the student a better understanding of the aspects developed in the expository classes, which will facilitate the preparation of the final exam.