ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Hours of tutorials: 1 Expository Class: 26 Interactive Classroom: 14 Total: 41
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Microbiology and Parasitology
Areas: Microbiology
Center Faculty of Biology
Call: First Semester
Teaching: With teaching
Enrolment: Enrollable
The general objective of the course is that the student will be able to identify the characteristics of viruses and distinguish the different replication cycles in host cells. They will also associate the main human diseases of viral origin with the causal agent and should be able to identify the typical symptomatology and the mechanisms that cause them, the mode of transmission of the diseases, the most suitable diagnostic methods and the therapeutic measures applicable in each case
Theory programme:
Topic I.- Introduction: History and definition of Virology. Viruses and evolution [0,5 h].
Topic II.- Viral nature [2h].
Topic III.- Study methods. [0,5h] (To be extended in tutorial)
Topic IV.- Viral Taxonomy [0,5h].
Topic V.- Viral replication [6h].
Topic VI.- Genetics of animal viruses [2h].
Theme VII.- Virus-cell and virus-host interactions [3h].
Theme VIII.- Infections of the Central Nervous System: Viral encephalitis and meningitis [2h].
Theme IX.- Respiratory infections [2h].
Topic X.- Gastrointestinal infections: [1h] Topic XI.
Epithelial and mucous membrane infections [2h] Track XI.- Epithelial and mucous membrane infections [2h] Track XII.
Topic XII.- Viral hepatitis. [1 h]
Haemorrhagic fevers and other geographically localised viral infections [2h] Topic XIII.- Haemorrhagic fevers and other geographically localised viral infections [2h] Topic XIV.
Acquired immunodeficiency syndrome and other retroviral diseases. [1 h]
Topic XV.- Infectious mononucleosis; Ocular and cardiac infections [1h].
Blackboard, Seminars and Tutorials Programme
Blackboard (online resolution)- Development of exercises and resolution of problems and practical cases related to different topics (methodology, structure, replication, genetics, diseases...).
Seminars: Presentation of work to promote the use of bibliography, interpretation and synthesis (4 hours).
Tutorials: Tutorials will be scheduled at the beginning of the course to study in depth the methodology of the study of viruses, and another one later on for the preparation of the seminars.
Practical programme
1. Cell cultures 2h
a. Types of cell culture media.
b. Visualisation of cultures and recognition of cell morphologies.
c. Subculture of cell mats
d. Viral inoculation and Visualisation of cytopathic effects
2. Viral Morphology and Ultrastructure 1h
a. Construction of icosahedral structures with models
b. Recognition of symmetry axes
c. Recognition of the significance of the triangulation factor.
3. Diagnosis: Physico-chemical characterisation 1.5h
a. Presence/absence of envelope: Treatment with lipid solvents, pH and temperature.
b. Genome type identification: Enzymatic and base analogue treatment.
4. Diagnosis and Typing: Serological methods 2h
a. Seroneutralisation for diagnosis
b. Seroneutralisation for typing (prcts. blackboard)
c. Immunodot
d. Immunofluorescence (prcts. slate)
e. ELISA
5. Diagnostics and Typing: Molecular Methods 3,5h
a. Nucleic acid hybridisation
b. PCR
c. Sequencing and phylogeny (blackboard prcts.)
Basic bibliography
Carrasco, L.,Almendral del Río, J.M. (coords., 2005. Virus patógenos. Madrid: Hélice.
Flint, J., Racaniello, V.R., Rall, G. F., Hatziioannou, T., Skalka, A.M. 2020. Principles of virology. Hoboken: Wiley
Shors, T., 2009. Virus: estudio molecular con orientación clínica. Buenos Aires: Editorial Médica Panamericana.
Storch, G.A., ed., 2000. Essentials of diagnostic virology. New York: Churchill Livingstone.
Strauss, J.H. and Strauss, L.G., 2008. Viruses and human disease. 2nd ed. Amsterdam: Elsevier Academic Press.
Complementary bibliography
Cann, A.J., 2009. Principios de virología molecular. Zaragoza: Acribia.
Carter, J.B. and Saunders, V., 2013. Virology: principles and applications. 2nd ed. Chichester, WS: John Wiley & Sons.
Collier, L. y Oxford, J., 2008. Virología humana: texto para estudiantes de medicina, odontología y microbiología. México: McGraw-Hill Interamericana.
Dimmock, N.J., Easton, A.J. and Leppard, K.N., 2007. Introduction to modern virology. 6th ed. Malden, MA: Blackwell Publishing.
Fenner, F, Bachmann, P.A., Gibbs, E.P.J., Murphy, F.A., Studdert, M.J. y White, D.O., 1992. Virología veterinaria. Zaragoza: Acribia.
Joklik, W.K., 1985. Virology. 2nd ed. Norwalk, CT: Appleton-Century-Crofts.
Knipe, D.M. and Howley, P.M. eds., 2001. Fields virology. 4th ed. 2 vol. Philadelphia: Lippincott Williams & Wilkins.
Levy, J.A., Fraenckel-Conrat, H. and Owens, R.A., 1994. Virology. 3rd. ed. Upper Saddle River, NJ: Prentice Hall.
Mettenleiter, T.C. and Sobrino, F. 2008. Animal viruses: molecular biology. Wymondham: Caister Academic Press.
Wagner, E.K., Hewlett, M.J., Bloom, D.C. and Camerini, D., 2008. Basic virology. 3rd. ed. Malden, MA: Blacwell Publishing.
Zuckerman, A.J. et al., 2009. Principles and practice of clinical Virology. 6th ed. West Sussex: Wiley
:
WEB pages
ICTV (International Commitee for Taxonomy of Virus): http://www.ncbi.nlm.nih.gov/ICTV/
The Big Picture Book of Viruses: http://www.virology.net/Big_Virology/BVHomePage.html
All the Virology on the WWW: http://www.virology.net/garryfavweb.htmlhttp://virologia.ua.es/dmsander….
Knowledge:
Con06 Understand the principles of bioenergetics and biosignalling, and know the main metabolic pathways and their regulation, as well as the flow of genetic and biochemical information in living beings.
Con07Know and understand the structure and function of viruses, prokaryotic and eukaryotic cells.
Skills/skills:
H/D01-Apply in an integrated way the theoretical-practical knowledge acquired in the transmission of information/ideas and in the approach and resolution of problems, both in academic and professional contexts.
H/D02-Knowing how to obtain and interpret relevant information and experimental results and to draw conclusions in subjects related to Biology.
H/D07-Ability to search for, process, analyse and synthesise information from different sources, including the use of ICT in the field of Biology.
H/D8-Ability to reason, argue and think critically
H/D10-Ability to work in groups and deal with problem situations in a collective way
H/D11-Ability to produce and present an organised and comprehensible text, adequately reflecting the sources of information used.
H/D12-Ability to make a clear, concise and coherent public presentation.
Competences:
Com01-That students have demonstrated possession, understanding and use of knowledge of the different areas of Biology, including some cutting-edge aspects of their field of study.
Com03-That students know how to apply their knowledge to their work or vocation in a professional manner and possess the abilities that are usually demonstrated through the elaboration and defence of arguments and the resolution of problems within their area of study.
Com04-To know and know how to apply instrumental techniques and design work protocols in the laboratory and in the field, applying the appropriate regulations and techniques related to safety, hygiene, waste management, quality and sustainable development.
The development of the subject in class, although it will be based, fundamentally, on the lecture, providing the concepts and illustrations of diagrams and photographs through audiovisual media, will encourage the active participation of the student through the formulation of questions or the posing of practical cases to solve in the classroom. The development of practical exercises and problem solving on line and in seminars will be promoted, as well as the preparation of specific works to promote the use of bibliography, interpretation and synthesis.
Compulsory activities: Laboratory practicals and theory exam.
Optional activities: Completion of case studies/problems (online) and seminar work (on-site defence).
Important. The evaluation will be based on the fulfilment of objectives, i.e. the maximum mark that the student aims to achieve.
The student must decide, and communicate to the teacher, the objectives he/she wishes to achieve at the beginning of the course. New objectives may be chosen at any time during the semester, but the completion of the training activities will be limited to those for which the period of execution has not yet ended. The mark obtained in these cases will be proportional to the exercises carried out.
Training activities
1. Compulsory activities
1.1. Demonstration of knowledge of the concepts of the subject.
Basic and compulsory activity
Maximum grade attainable 6
- Theoretical exam* (The student's theoretical knowledge will be assessed by defining basic concepts, answering short questions, interpreting images and diagrams, solving practical cases and developing fundamental topics. In addition to the contents, the rigour, clarity, conciseness and elegance of the exposition will be taken into account): 60% of the overall mark.
[Con06, Con07; H/D01, H/D08, H/D10; Com01, Com03; ]
1.2. Performance of the practicals
Compulsory activity
Maximum grade achievable 1
- Evaluation of the practicals10% of the overall mark**.
There are two options to choose from. The option chosen must be communicated to the teacher within the indicated period.
1.2.1. Assessment of the practical report (the presentation, the quality of the report and the description and explanation of the tests carried out shall be taken into account).
1.2.2. Completion of a practical examination
H/D01, H/D02, H/D10; Com04] [H/D01, H/D02, H/D10; Com04] 2.
2. Optional activities
2.1. Resolution of practical cases/problems***:
Adds a maximum of 1.5 points to the mark obtained in the examination.
- Evaluation of the resolution of exercises, problems and practical cases: The presentation and reasoning in the resolution will be taken into account and will account for 15% of the overall mark.
[H/D01, H/D07, H/D08, H/D10, H/D11; Com3].
2.2. Search for, analysis and elaboration of information***.
Adds a maximum of 1.5 points to the mark obtained in the examination.
- Presentation and memory of seminar work (the quality of the memory and presentation will be taken into account, as well as the confidence in answering the questions posed): 15% of the overall mark.
[H/D01, H/D02, H/D07, H/D08, H/D10, H/D11, H/D12, Com1, Com3].
* The theory exam will be taken in writing, in accordance with the date established by the dean's team, as published in the student guide of this Faculty. Optionally, students who so wish may take the oral exam, on a date previously agreed with the interested party(s). The mark for the theory exam can only be maintained until the make-up exam in July of the current academic year. It will be necessary to pass the theory exam (5) in order to add up the rest of the marks.
** Completion of the practicals is a prerequisite for taking the theory exam. The internship grade will be maintained for two academic years if the student so requests.
*** Recoverable items: Students who have to sit the make-up exams may ask their teachers to assign them the tasks corresponding to this section, in order to achieve the percentage of evaluation corresponding to seminars and exercises.
CLASSROOM WORK
Lectures, 26h
Laboratory practicals, 9h
Blackboard/seminar practicals, 4h
Tutorials in small groups or one-to-one, 1h
Exams, 2 hours
TOTAL HOURS OF CLASSROOM WORK: 42H
PERSONAL WORK OF THE STUDENT
Individual study, 45.5h
Elaboration of practical reports, 6h
Preparation of assignments, 11h
Recommended readings, library activities or similar, 2h
Preparation of syntheses of topics, 5h
TOTAL STUDENT PERSONAL WORK HOURS: 69,5h
The estimated number of non face-to-face hours that the student will have to apply for the correct learning of the subject should be 1.5 equivalent to the face-to-face hours, especially for the theoretical classes. As for the practical programme, the classroom hours will be necessary, with continuous assessment through personalised monitoring, as well as the presentation of a practical report in which the student must record the procedures carried out and incidents that have taken place during its development. The student's participation in their own learning through the resolution of exercises, problems and practical cases, as well as in the development of seminar work, will be taken into account.
Follow the recommendations of the teachers during the academic course
Knowledge of microbiology, biochemistry and genetics is required for the correct learning of the subject.
The subject will have a virtual classroom on the Moodle platform in which, in addition to the contents developed by the teachers, other useful information will be available to the students.
n cases of fraudulent performance of exercises or tests, the provisions of the ‘Regulations on the evaluation of students’ academic performance and review of grades’ shall apply.
Carlos Pereira Dopazo
Coordinador/a- Department
- Microbiology and Parasitology
- Area
- Microbiology
- Phone
- 881816083
- carlos.pereira [at] usc.es
- Category
- Professor: University Lecturer
Maria Isabel Bandin Matos
- Department
- Microbiology and Parasitology
- Area
- Microbiology
- Phone
- 881816087
- isabel.bandin [at] usc.es
- Category
- Professor: University Lecturer
Emma Gonzalez Rosales
- Department
- Microbiology and Parasitology
- Area
- Microbiology
- emmagonzalez.rosales [at] usc.es
- Category
- Ministry Pre-doctoral Contract
| Monday | |||
|---|---|---|---|
| 10:00-11:00 | Grupo /CLE_01 | Spanish | Classroom 09. Barbara McClintock |
| Tuesday | |||
| 10:00-11:00 | Grupo /CLE_01 | Spanish | Classroom 09. Barbara McClintock |
| 01.17.2025 10:00-14:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
| 01.17.2025 10:00-14:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |
| 06.18.2025 10:00-14:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |