ECTS credits ECTS credits: 6
ECTS Hours Rules/Memories Student's work ECTS: 99 Hours of tutorials: 3 Expository Class: 24 Interactive Classroom: 24 Total: 150
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Functional Biology
Areas: Plant Physiology
Center Faculty of Biology
Call: First Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable
The aim of the subject is for students to acquire the following skills:
- Detail some applications and the current state of the different fields of plant biotechnology.
- List and know how to apply different techniques of propagation and preservation by in vitro culture, including cell cultures.
- Identify the different processes that give rise to a phenotype caused by a genetic manipulation, from the role of the promoters to the alteration of metabolic flows.
- Know how to make different types of genetic constructions for overexpression, silencing and genome editing.
- List and know how to apply different techniques of stable and transient transformation by means of Agrobacterium or biolistic transformation, including the transformation of chloroplasts.
LECTURES:
1. Introduction: Applications and current state of plant biotechnology, 2 h
2. Applications of biotechnology in plant breeding: Selection systems, markers, heterosis and genomics, 3 h
3. In vitro culture: Propagation Techniques, conservation methods, cell cultures and applications, 5 h
4. Functional genomics: Identification of candidate genes and functional characterization, 4 h
5. Genetic manipulation: Design of strategies from the gene to the phenotype, 6 h
6. Construction design: overexpression, silencing and gene editing, 3 h
7. Methods of transformation and selection, 4 h
LABORATORY:
The students will work 7 hours in the laboratory to complete the following activities:
1. In vitro culture
2. Transient transformation.
In addition, students will carry out exercises based on the application of bioinformatic tools with the help of tutorials.
In scenario 3, in person laboratory classes will be replaced by additional exercises based on the analysis of protocols and results.
SEMINARS:
Discussion of scientific papers related to the theoretical contents of the subject.
TUTORIALS:
Orientation to the students in the preparation of laboratory reports and presentations.
BASIC
Stewart N. ed., 2012. Plant biotechnology and genetics: principles, techniques, and applications. 2nd ed. Hoboken: John Wiley & Sons Inc.
Altman A., Hasegawa P. M. eds., 2012. Plant biotechnology and agriculture: prospects for the 21st century. Amsterdam: Academic Press.
Plant Biotechnology Journal. https://onlinelibrary.wiley.com/journal/14677652
ADDITIONAL
Slater A., Scott N. W., Fowler M. R. eds., 2008. Plant biotechnology: the genetic manipulation of plants. Oxford: Oxford University Press.
In this course the students will work on the following competencies:
BASIC AND GENERAL
As detailed in the Degree memorandum.
TRANSVERSAL
CT1 – Integrative thinking and ability to approach problems from different perspectives.
CT2 - Search, process, analyze and synthesize information from various sources.
CT3 -Organize and plan their work.
CT4 -Interpret experimental results and identify consistent and inconsistent elements.
CT5 -Work as a team.
CT6 - Reason critically.
CT7 - Maintain an ethical commitment.
CT8 -Adapt to new situations (resilience).
SPECIFIC
CE3- Be familiar with and know how to apply instrumental techniques and protocols in a laboratory, applying the regulations and procedures related to safety and hygiene, waste management and quality control.
CE9- Be able to identify the different types of viruses, microorganisms and animal and plant tissues, understand their development, organization and physiology and know their application in the field of Biotechnology
CE11- Know the molecular basis and the techniques to manipulate the genetic information of microorganisms, animals and plants and know how to apply those techniques in different biotechnological applications.
In scenarios 2 and 3, Microsoft Teams will be used preferentially to communicate with the students.
Lectures will be taught in 27 one-hour sessions, with a frequency of 2 hours per week. They will cover the basic aspects of the different topics that make up the program of the subject. Competencies developed: CG1, CG2, CG3, CB1, CB2, CB3, CT1, CT2, CT4, CT6, CE4, CE9 and CE11.
In scenario 2, depending on the situation and the availability of infrastructure, lectures may be carried out partially (shifts) or fully online, preferentially using the Teams platform. In scenario 3 all lectures will be online. In the case of fully online lectures, pre-recorded classes will be provided and will be complemented with doubt-solving sessions by videoconference during the lecture class hours. In all scenarios, students will have recordings of the classes (audio or video).
Seminar classes will be taught in small groups over 8 sessions. In these classes, students must prepare and present a research project to solve a problem using the techniques studied in the subject. Competencies developed: CG1, CG3, CG4, CG5, CB1, CB2, CB3, CB4, CB5, CT1, CT2, CT4, CT5, CT6, CE4, CE9 and CE11.
In scenario 2, depending on the situation and the availability of infrastructure, seminar classes may be carried out partially (shifts) or fully online, through videoconferences on the Teams platform. In scenario 3 seminars will be online.
In scenarios 1 and 2, laboratory classes will be carried out in 7 hours distributed in 3 sessions, complemented with 7 additional hours of autonomous exercises. Students must prepare an individual report that will include the design of a protocol and the analysis and discussion of the results. Competencies developed: CG1, CG2, CG3, CG4, CG5, CB1, CB2, CB3, CB4, CB5, CT3, CT4, CT5, CT6, CT7, CT8, CE3
In scenario 3, lab classes will be replaced by additional exercises to learn how to analyze protocols and results that will be carried out through videoconferences in Teams.
Throughout the course there will be 3 60-minute tutoring sessions in very small groups in which the use of bioinformatics resources will be explained, and exercises will be reviewed. Competencies developed: CG1, CG2, CG3, CG4, CG5, CB1, CB2, CB3, CB4, CB5, CT1, CT2, CT3, CT4, CT5, CT6, CT7, CT8, CE3, CE4, CE9 and CE11.
Tutorials will be carried out online through video conferences in Teams in all scenarios.
REQUIRED ACTIVITIES THAT ARE NOT EVALUATED:
It will be necessary to attend the 3 laboratory classes to submit the laboratory report.
.REQUIRED ACTIVITIES THAT ARE EVALUATED:
- Laboratory report (20% of the final grade). Competencies evaluated: CG1, CG2, CG3, CG4, CG5, CB1, CB2, CB3, CB4, CB5, CT3, CT4, CT5, CT6, CT7, CT8, CE3
It will be necessary to present a lab report and obtain a minimum score of 4 out of 10 to be able to take the final exam. In case of obtaining at least a 5 and failing the subject, it will not be necessary to repeat the laboratory classes and the grade of the report will be kept in the following two courses. In order to submit a new lab report, it will be necessary to attend at least the two computer practical sessions. Students that do not submit the laboratory report will be considered not evaluated.
- Presentation and discussion of papers (30% of the final grade). Competencies evaluated: CG1, CG2, CG3, CG5, CB1, CB2, CB3, CB4, CB5, CT1, CT2, CT4, CT6, CE4.
The evaluation of the presentation and discussion of papers will take place in person in scenario 1 and online in scenario 3. In scenario 2 the evaluation will be in person if it is possible to present the papers in person and online otherwise. A minimum grade of 4 out of 10 will be required to sit for the final exam. In case of obtaining at least a 5 and failing the subject, it will not be necessary to repeat the presentation and the grade of the report will be kept in the following two courses. In these cases, the presentation will be voluntary.
- Final exam (50% of the final grade). Competencies evaluated: CG1, CG2, CG3, CG4, CG5, CB1, CB2, CB3, CB4, CB5, CT1, CT2, CT4, CT6, CE4.
The final exam will consist of solving plant biotechnology problems. During the final exam student notes can be consulted. If possible, it will be done in person. In scenario 3 or in case that in person exams are not allowed in scenario 2, it will be done online.
IMPORTANT: For cases of fraudulent performance of exercises or tests, the provisions of the “Normativa de avaliación do rendemento académico dos estudantes e de revisión de cualificacións” will apply.
WORK IN THE CLASSROOM:
- Lectures: 27 hours
- Interactive seminars: 7 hours
- Laboratory classes and additional exercises: 14 hours
- Tutorials in small groups: 3 hours
- Final exam: 3 hours
PERSONAL STUDENT WORK:
- Individual study and preparation of the lab report: 96 hours
TOTAL WORK HOURS: 150 HOURS
- It is advisable to study the subject during the whole course.
- It is important to organize the class notes so that they can be consulted efficiently during the seminars and exams.
The subject material and all announcements will be available in the virtual classroom. A Teams group will be used to share the results of the laboratory classes.
Maria Del Carmen Rodriguez Gacio
- Department
- Functional Biology
- Area
- Plant Physiology
- mdelcarmen.rodriguez.gacio [at] usc.es
- Category
- Professor: Temporary PhD professor
Javier Sampedro Jiménez
Coordinador/a- Department
- Functional Biology
- Area
- Plant Physiology
- Phone
- 881813113
- javier.sampedro [at] usc.es
- Category
- Professor: Temporary PhD professor
Marcos Viejo Somoano
- Department
- Functional Biology
- Area
- Plant Physiology
- Phone
- 881813322
- marcos.viejo [at] usc.es
- Category
- Professor: LOU (Organic Law for Universities) PhD Assistant Professor
| Monday | |||
|---|---|---|---|
| 10:00-11:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |
| Tuesday | |||
| 09:00-10:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |
| Wednesday | |||
| 12:00-13:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |
| 01.18.2024 10:00-14:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |
| 06.19.2024 10:00-14:00 | Grupo /CLE_01 | Classroom 03. Carl Linnaeus |