ECTS credits ECTS credits: 3
ECTS Hours Rules/Memories Student's work ECTS: 45 Hours of tutorials: 2 Expository Class: 18 Interactive Classroom: 10 Total: 75
Use languages Spanish, Galician
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Center Faculty of Biology
Call: Second Semester
Teaching: With teaching
Enrolment: Enrollable | 1st year (Yes)
Introduce students to the study of neuronal physiology
Teach the functioning of individual neurons
Match the membrane properties and ion channels with neuronal behavior
Introduce students to the study of channelopathies
Introduce students to techniques for the study of individual neurons (patch-clamp)
Item 1. A brief history. Surgical Papyrus: Imhotep and Edwin Smith. The animal electricity: Galvani and Volta. Matteucci and du Bois. The neuron: Golgi and Cajal. Electroencephalogram: Caton and Berger. Individual Registration: Eccles. The axon of the squid: Hodgkin and Huxley. Ion Channels: Neher and Sakmann.
Item 2. Voltage control technique. Classical voltage-clamp technique. Ionic basis of the action potential. Ion substitution experiments. Current-voltage curves. Reversal potential.
Item 3. Patch-clamp technique. Patch-clamp modes. Bath and pipette solutions. Basic patch equipment. Problems Associated with Patch. Utilities of the patch technique. Preparations for Patch. Techniques related and associated with patch-clamp. News of the technique.
Item 4. Voltage-dependent sodium channel. Types. Structure. Electrical properties. Pharmacology. Function. Related Channelopathies.
Item 5. Voltage-dependent potassium channels. Type: Delayed Rectifier, transient A-type K channels, M-Channels. Structure. Electrical properties. Pharmacology. Function. Related Channelopathies.
Item 6. Calcium dependent potassium channels. Types: Low conductance calcium-dependent potassium channels (SK type). Intermediate conductance channels (IK). High conductance calcium-dependent potassium channels (BK type). Structure. Electrical properties. Pharmacology. Function. Related Channelopathies.
Item 7. Leakage potassium channels (K2P). Types. Structure. Electrical properties. Pharmacology. Function. Related Channelopathies.
Item 8. Inwardly rectifying potassium channels (Kir). Types. Structure. Electrical properties. Pharmacology. Function. Related Channelopathies. Beta cells and insulin secretion
Item 9. Voltage-dependent calcium channels. Types. Structure. Electrical properties. Pharmacology. Function. Related Channelopathies. Low-threshold T-type Calcium channel. High-threshold L, N, P / Q and R calcium channels.
Item 10. Ligand-gated channels. Types. Structure. Electrical properties. Pharmacology. Function. Related Channelopathies. Nicotinic acetylcholine receptors. Ionotropic glutamate receptors. GABA receptors.
PRACTICES
Practice 1. Study of the role of ion channels in the neuronal behavior. Current clamp experiments simulated by computer. We use the Neuron program of Huguenard and McCormick.
Practice 2. Study of ionic currents across the membrane. Voltage clamp experiments simulated by computer. We use the Neuron program of Huguenard and McCormick
-Aidley and Stanfield (1996) Ion channels. Molecules in action. Cambridge University Press
-Ashcroft FM (2000) Ion channels and disease. Academic Press
-Frumento (1995) Biofísica. Mosby-Doyma
-Hammond (2008) Cellular and molecular neurophysiology. Academic Press
-Hille B (2001) Ion channels of excitable membranes. Sinauer Associates
-Huguenard and McCormick (1994) Electrophysiology of the neuron. An interactive tutorial. Oxford University Press
-Kandel, Schwartz y Jessell (2000) Principios de neurociencia. McGraw-Hill-Interamericana.
-Molleman (2003) Patch Clamping. An introductory guide to patch clamp electrophysiology. Wiley
-Rudy and Iverson (1992) Ion Channels. In Methods in Enzymology vol. 207. Academic Press.
-Sakmann and Neher (1995) Single-Channel recording. Plenum Press.
-Wallis (1993) Electrophysiology. A practical approach. Oxford University Press
- Knowledge of neuronal physiology
- Knowing how individual neurons work
- Be able to relate the properties of the membrane and ion channels in neuronal behavior
- General knowledge in the study of channelopathies
- Learning techniques to the study of individual neurons (patch-clamp).
Lectures
Interactive classes: Seminars or practices
Tutoring in small groups or individualized
Evaluation Activities
- Attendance at lectures and practicals is mandatory
- There will be one final exam. This will consist of 50 multiple choice questions with 4 options, wrong answers will have negative consequences. The test will include questions about the practicals. The exam can get a score between 0 and 10.
-Several activities will be positively valued (between 0.5 and 1 point per activity):
1. Written work to be performed during the development of the practicals. Answer to questions in the script. Individual Problem Resolution.
2. Active participation in the lectures and practicals, tutorials, scientific meetings, seminars, conferences etc. will be evaluated positively.
3. Attending a real experiment in the laboratory shall be optional but is taken into account for the final score.
- It is required to obtain a minimum of 5 out of 10 to pass the subject
Lectures: 16 h.
Interactive classes: Seminars or practices: 10 h.
Tutoring in small groups or individualized: 2 h
Evaluation Activities: 2 h.
Individual Study: 30 h
Developing practical memories, jobs: 15 h
- Active participation in lectures and practicals
- Daily study of the content presented in class
- Regular use of at least one of the guide books
- Attendance at tutorials for clarification
- Search for additional information (personal) in books and internet