ECTS credits ECTS credits: 6
ECTS Hours Rules/Memories Hours of tutorials: 1 Expository Class: 36 Interactive Classroom: 17 Total: 54
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Biochemistry and Molecular Biology
Areas: Biochemistry and Molecular Biology
Center Faculty of Biology
Call: First Semester
Teaching: With teaching
Enrolment: Enrollable
- Know the molecular basis of genetic information, delving into the study of aspects related to the structure of the genome in cells and its importance in the control of gene expression.
- Identify the relationship between genes and genomes and their importance in organisms.
- Know the mechanisms by which cells transmit information from the environment that allows cells to generate an ideal response to a changing environment, delving into the study of transduction routes activated by different types of signals.
- Know the specific mechanisms by which gene expression is controlled at different levels, especially in higher organisms and in complex processes such as cell division and death.
- Acquire the ability to assess the current state of scientific knowledge of the different topics of Molecular Biology.
- Know the Molecular Biology techniques that allow the study of the structure, function and regulation of nucleic acids and proteins in cells.
• EXPOSITIVE CLASSES (Theory Program, 36 Face -to Face hours)
I. THE STRUCTURE OF THE GENOME (10h).
Lesson 1. Molecular Biology and its origins. Nucleic acids DNA and RNAs (1.5h).
Lesson 2. Eukaryotic DNA Condensation: DNA Supercoils and Nucleosomes (3.5h).
Lesson 3. Organization of eukaryotic DNA: Chromatin and chromosome structures (2.5h).
Lesson 4. Genes and genomes (2.5h).
II. THE EXPRESSION OF THE GENOME AND ITS REGULATION (26h).
(A) CELL SIGNALING AND GENE EXPRESSION (14h).
Lesson 5. General principles and elements of cell signaling. General mechanisms of transduction by external and internal signals (2h).
Lesson 6. Proteins in cell signaling: transducing proteins, protein interactions and gene regulatory proteins (3h).
Lesson 7. Intracellular signaling pathways activated through G protein-coupled receptors (GPCRs) (2h).
Lesson 8. Intracellular signaling pathways activated by mitogenic factors, cytokines and receptors with kinase activity (3.5h).
Lesson 9: Bidirectional signaling, proteolytic pathways and lipid signaling (1.5h)
Lesson 10. Intracellular signaling pathways activated by hydrophobic signals (2h).
(B) THE REGULATION OF THE EXPRESSION OF THE EUKARYOTE GENOME (12h).
Lesson 11 Regulatory RNAs and regulation of gene expression (2.5h).
Lesson 12: Gene control. Epigenome: Chromatin structure and control of gene expression (2.5h).
Lesson 13. Regulation of gene expression I: Transcriptional control (3.5h).
Lesson 14. Regulation of gene expression II: Transport, localization and stability of mRNA and translational control (3.5h).
• INTERACTIVE CLASSES (17h; Face -to Face)
Interactive seminars (12h in person).
They aim to promote learning through the student's participation in the class and to reinforce/ deepen
specific aspects of the subject (see teaching and evaluation methodology) that also allow its continuous
evaluation.
Practical seminars (5 hours in person): Two sessions of compulsory and evaluable attendance:
- Session I (laboratory; 2.5h). Extraction and experimental analysis of a bacterial plasmid by gel electrophoresis.
- Session II (computer classroom; 2.5h). Analysis and visualization of cis and trans elements involved in gene
expression through databases and bioinformatics applications.
• TUTORIAL CLASSES (1h)
At the beginning of the course, it will be used for the detailed presentation of the subject and the teaching guide.
• EXAM (3 contact hours)
BASIC BIBLIOGRAPHY
- Alberts, B. et al., 2022. Molecular Biology of the Cell. 6th ed. New York: W.W. Norton & Company.
- Hérraez, A., 2012. Texto ilustrado e interactivo de Biología Molecular e Ingeniería Genética. Conceptos, técnicas y
aplicaciones en ciencias de la salud. 2ª ed. Barcelona: Elsevier.
Available online: https://iacobus.usc.gal/permalink/34CISUG_USC/tmlevo/alma99101338132920…
- Kraus. G., 2014. Biochemistry of Signal Transduction and Regulation. 5th ed. Weinheim: Wiley-VCH.
- Lodish, H., Berk, A., Kaiser, C.A., Krieger, M., Brestscher, A., Ploegh, H., Martin, K.C., Yaffe, M.B., Amon, A. 2021.
Molecular Cell Biology. 9th ed. New York: Macmillan International.
Available online through the Editorial Panamericana collection:
https://iacobus.usc.gal/permalink/34CISUG_USC/1oc2t8m/alma9910135440500…
- Nelson, D.L. & Cox, M.M., 2021. Lehninger. Principles of Biochemistry. 8ª ed. New York: Macmillan International.
- Watson, J.D. et al., 2016. Biología molecular del gen. 7ª ed. Buenos Aires: Ed. Médica Panamericana
Available online through the Editorial Panamericana collection:
https://iacobus.usc.gal/permalink/34CISUG_USC/o7pcup/alma99101338258140…
COMPLEMENTARY BIBLIOGRAPHY
- Brown, T.A., 2018. Genomes 4. 4th ed. New York: Garland Science.
Fifth edition available online:
https://iacobus.usc.gal/permalink/34CISUG_USC/o7pcup/alma99101346385430…
- Clark, D.P. and Pazdernik, N.J., 2019. Molecular Biology. 3nd ed. Boston: Academic Press.
Available online: https://iacobus.usc.gal/permalink/34CISUG_USC/o7pcup/alma99101353221490…
- Craig, N.L. et al., 2014. Molecular Biology. Principles of Genome Function. 2nd ed. Oxford: Oxford University Press.
- Latchman, D. S., 2015. Gene Control. 2nd ed. New York: Garland Science.
- Marks, F., Klingmüller, U., Müller-Decker, K., 2017. Cellular Signal Processing. 2nd ed. New York: Garland Sciences.
LIST OF LEARNING OUTCOMES
Knowledge/contents: Con01, Con03, Con05, Con06, Con09
Skills/abilities: H/D01, H/D02, H/D08, H/D010, H/D011, H/D012
Competencies: Comp01, Comp03, Comp04, Comp06
• Expository classes: non-obligatory face-to-face activity in the format of master classes for the presentation of the lessons of the theoretical program of the subject with the use of blackboard and supported by computer presentations.
• Interactive classes. They are face-to-face, evaluable and not mandatory.
• Learning will be promoted through problem solving and the active participation of the student by responding (orally or in writing) to different types of questions (see evaluation system). They will also allow you to complete and strengthen specific aspects developed in the expository classes that have a special interest, as well as reinforce and review key concepts of the subject.
• Practical Classes: Mandatory and evaluable.
• Two types of representative practical activities will be carried out to complement the theoretical knowledge acquired in the expository classes:
• Practical session (2.5h) of extraction of a bacterial plasmid and its experimental analysis, in which students will previously have a practice script.
• Bioinformatics session (2.5h), in which some databases will be managed for information search and DNA analysis, modeling program for 3D spatial analysis (visualization of DNA fragments linked to specific proteins). Finally, they will answer another very short written questionnaire.
• Tutorial: at the beginning of the course, it will be used for the detailed presentation of the subject and the teaching guide.
• Exam. In-person and mandatory on the expository syllabus that will include questions from the syllabus in different formats (test type, relationship of concepts, theoretical questions and reasoning questions...).
LEARNING ASSESSMENT SYSTEM
The evaluation of learning will be done in person and will consist of:
(a) Evaluation of expository classes (Final exam).
Mandatory evaluable activity complementary to continuous evaluation.
The contents of the theoretical program will be evaluated through a written exam that will contain different types of questions (test, relating concepts and reasoning and argumentation). The maximum grade obtained in the exam (10 points) is equivalent to 75% of the final grade, as long as the conditions indicated below are met (see final evaluation).
(b) Evaluation of interactive classes (Continuous evaluation).
- The seminars will be evaluated through the participation of the student, evaluating their oral responses to identify, interpret, describe and explain images/figures/schemes related to the contents of the expository classes or responding (orally or in writing) to issues/questions/ exercises proposed (by the teacher) in the class itself and/or through questionnaires, previously accessible to students through the virtual classroom. The evaluation could also involve the presentation (delivery) and/or exhibition of works in order to deepen or reinforce specific concepts and topics of the subject.
It will be graded from 0 to 10 points and the grade obtained will represent 15% of the final grade of the subject (maximum 1.5 points of the grade of the subject, as long as it meets the requirements indicated below (see final evaluation). .
- (c) Laboratory practical classes and computer classroom classes (Continuous evaluation).
. - The execution of the practice or the activities proposed in the bioinformatics classroom will be evaluated, with a maximum of 0.5 points. The resolution of the questionnaires and/or delivery of tasks will be valued with another 0.5 points maximum.
- The final grade for the practical activities will be the sum obtained in the execution, resolution and delivery of questionnaires or tasks, with a maximum value of 1 point, which represents 10% of the final score for the subject).
(C) Final evaluation.
- The final grade will be obtained as the weighted average of the partial grades obtained in (a), (b) and (c), which represent, respectively, 75%, 15% and 10% of the final grade and as long as has achieved in the qualification of (a) a minimum of 4.7 points and the minimum answer requirement (85% of the exam questions answered). The exam must also be compensated, in terms of the grade obtained in answers to objective questions (test type...), with respect to that achieved in questions whose answer involves relating and/or arguing/reasoning concepts.
- The lack of qualification in (b) will imply that the student renounces that part of the grade, so that he could obtain, at most, 8.5 points in the final grade, in the event that both in the content exam of the expository classes as in the practical one would have achieved 10 points out of 10.
- In the second call, the evaluation dependent on the exam will be repeated exclusively and, if it has not been done previously, also the exam corresponding to the seminars, which will be voluntary. In any case, students will only be evaluated for the seminars once per course. The percentages for each part of the evaluation will be the same as those indicated above.
- Repeaters
- They will be exempt from doing the practices, but the grade from the seminars from the previous year will not be retained.
LEARNING EVALUATION SYSTEM
(A) FINAL EXAM
Knowledge/Contents: Con01, Con05, Con06, Con09
Skills/Abilities: H/D01, H/D02, H/D08, H/D011
Competencies: Comp01, Comp03, Comp06
(B) CONTINUOUS EVALUATION
(a) Seminars:
Knowledge/ Contents: Con01, Con03, Con05, Con06, Con09
Skills/Abilities: H/D01, H/D08, H/D012
Competencies: Comp01, Comp03, Comp06
(B) Laboratory Practices
Skills/Abilities: H/D01, H/D02, H/D08, H/D010, H/D011
Competencies: Comp01, Comp03, Comp04, Comp06
PRESENTIAL WORK IN THE CLASSROOM
Theoretical teaching (expository classes): 36h
Interactive teaching (classroom seminars): 12h
Interactive teaching (laboratory/computer classroom): 5h
Group tutoring: 1h
Exam: 3h
PERSONAL WORK
Individual Study: 93h
• Attendance at all teaching activities.
• Use of the recommended bibliography and what was presented in the virtual classroom, if applicable.
• Participation in the corresponding classes, responding orally to the issues/questions posed by the teacher, both in the class itself and in the specific ones of the seminars.
• Make summaries/schemes or scripts that synthesize the important contents of each of the program lessons, trying to combine and relate different aspects that are explained in each of them.
• Prepare map(s) that allow gathering central metabolic pathways in different situations in order to visualize, relate, visualize and clarify aspects of the subject explained in separate blocks.
• Self-assessment of understanding of the contents. The student should try to generate, by himself and using books, especially those recommended in the bibliography, a catalog of questions and answers so that he can face the exam with more chances of success.
• Make use of tutorials for any type of question related to the subject.
• The contents, order and time dedicated to each of the topics may be modified, with respect to the program presented in the teaching guide, by the incorporation of new concepts and more recent updates to it.
• It is recommended that for a better understanding of the subject you have basic prior knowledge of subjects such as biochemistry I, biochemistry II, genetics I/II, cell biology, animal and plant physiology, microbiology, as well as basic notions of chemistry (organic and inorganic).
• The information available to the student will be left through the virtual classroom and contact/communication with the student will be established through email and/or the MS-Teams platform.
• In cases of fraudulent completion of exercises or tests, the provisions of the "Regulations for evaluating students' academic performance and reviewing grades" will apply.
• It is expressly prohibited for students to distribute, among people outside the course, the teaching material (both written and audiovisual) available on the Virtual Campus or provided by the teaching staff through other channels.
Primitivo Barja Francisco
Coordinador/a- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881816931
- primi.barja [at] usc.es
- Category
- Professor: University Lecturer
Maria Lourdes Dominguez Gerpe
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- ml.dominguez.gerpe [at] usc.es
- Category
- Professor: Temporary supply professor to reduce teaching hours
Alvaro Duran Bravo
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- alvaroduran.bravo [at] usc.es
- Category
- Ministry Pre-doctoral Contract
Maria Blanquer Garate
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- maria.blanquer.garate [at] usc.es
- Category
- USC Pre-doctoral Contract
| Wednesday | |||
|---|---|---|---|
| 12:00-13:00 | Grupo /CLE_01 | Spanish | Classroom 01. Charles Darwin |
| 13:00-14:00 | Grupo /CLE_02 | Spanish | Classroom 02. Gregor Mendel |
| Thursday | |||
| 12:00-13:00 | Grupo /CLE_01 | Spanish | Classroom 01. Charles Darwin |
| 13:00-14:00 | Grupo /CLE_02 | Spanish | Classroom 02. Gregor Mendel |
| Friday | |||
| 12:00-13:00 | Grupo /CLE_01 | Spanish | Classroom 01. Charles Darwin |
| 13:00-14:00 | Grupo /CLE_02 | Spanish | Classroom 02. Gregor Mendel |
| 12.20.2024 10:00-14:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
| 12.20.2024 10:00-14:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |
| 12.20.2024 10:00-14:00 | Grupo /CLE_01 | Classroom 03. Carl Linnaeus |
| 06.13.2025 10:00-14:00 | Grupo /CLE_01 | Classroom 01. Charles Darwin |
| 06.13.2025 10:00-14:00 | Grupo /CLE_01 | Classroom 02. Gregor Mendel |