ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Student's work ECTS: 76.5 Hours of tutorials: 4.5 Expository Class: 13.5 Interactive Classroom: 18 Total: 112.5
Use languages Spanish, Galician
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Center Higher Technical Engineering School
Call: First Semester
Teaching: Sin Docencia (En Extinción)
Enrolment: No Matriculable (Sólo Planes en Extinción)
To provide knowledge of biology, especially of biochemistry and molecular biology, by means of procedures of study, experience and practice, and encouraging critical thinking, in order that the futures Master in Chemical Engineering and Bioprocess could develop their professional skills related to the biotechnological area.
UNIT 1. General introduction to Biochemistry and Molecular Biology. Estructure and function of biomolecules. Transduction and energy storage. Metabolism overview.
UNIT 2. Introduction to the metabolism. Concept of metabolism. Types of routes and metabolic reactions. Bioenergetics.
UNIT 3. Enzymes. Characteristics of the enzymatic reaction. Kinetic Mm. Enzyme inhibition. Concept of alosterism. Regulation of the enzymatic activity.
UNIT 4. Central metabolic pathways and energetic metabolism. Example: bioethanol production in Saccharomyces cerevesiae and Zymomonas mobilis. Brief summary of other metabolic pathways and their integration in the central pathways.
UNIT 5. Metabolism integration. Integration of metabolic pathways. Production of secondary products. Regulation of metabolism.
UNIT 6. Central Dogma of Molecular Biology. DNA structure. Genes and Genomes. DNA expression: transcription and translation. Genetic Code and mutations.
UNIT 7. Regulation of Gene Expression. Regulation of gene expression in prokaryotes: the lac operon. Regulation of gene expression in eukaryotes.
UNIT 8. Genetic engineering. Manipulation and analysis of DNA molecules. DNA sequencing. PCR. Cloning. Vector types. Transforming prokaryotic and eukaryotic cells.
UNIT 9. Applications of Bioengineering. Genetically modified organisms. Production of recombinant proteins. Examples of Metabolic engineering.
Students will have available (both in the laboratory and in the practice manual) all the information about the potential risks of the practices (handling and disposal of biological material, flammable and / or toxic products, materials at high temperatures) and necessary PPE (lab coat, safety goggles, chemical and thermal protection gloves).
PRACTICES 1-2: Determination of an enzymatic activity. Effect of diverse effectors and inhibitors. Calculation of kinetic parameters.
PRACTICES 3-4: Cloning of a cDNA. Bacterial transformation and plasmid isolation from bacterial cultures. Analysis of amplified cDNA by agarose gel electrophoresis of the purified plasmid and digested with restriction enzymes.
BASIC BIBLIOGRAPHY
CLARK, D.P. and PAZDERNIK, N.J., 2016. Biotechnology. [on line] 2nd ed. Amsterdam: Elsevier Academic Press. Available at: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780123850157/biotech…
-McKee, T. and McKee, J. R. 2020. Bioquímica. Las bases moleculares de la vida. 7ª ed. McGRAW-HILL Interamericana Editores. [on-line]https://accessmedicina-mhmedical-com.ezbusc.usc.gal/book.aspx?bookid=29…
COMPLEMENTARY BIBLIOGRAPHY
FEDUCHI CANOSA, E. e outros. Bioquímica. Conceptos esenciales. 2ª edición. Madrid: Editorial Panamericana, 2015. ISBN: 978-84-9835-875-9
GLICK, B. R., J.J PASTERNAK and C.L. PATTEN, C. L. Molecular biotechnology: principles and applications of recombinant DNA. 4ª edición. Washington D.C; ASM Press, 2010. ISBN: 978-1-55581-498-4
HERRÁEZ SÁNCHEZ, Á. Texto ilustrado e interactivo de biología molecular e ingeniería genética: conceptos, técnicas y aplicaciones en ciencias de la salud. 2ª edición. Barcelona: Elsevier, 2012. ISBN: 978-84-8086-647-7
NELSON, D. L. and M.M. COX. Lehninger Principios de Bioquímica. 6ª edición. Barcelona: Editorial Omega, 2014. ISBN: 978-84-282-1603-6
TYMOCZKO, J. L., J.M. BERG and L. STRYER. Bioquímica: Curso básico. 2ª edición. Barcelona: Editorial Reverté, 2014. ISBN:788429176032
OKAFOR, N. Modern industrial microbiology and biotechnology. Enfield (NH): Science Publishers, 2007. ISBN: 1578084342, 9781578084340
To help meet the skills included in the degree repor tCB6, CB7, CB10, CG7, CE1, CE3, CT4 y CT5. To a greater extent the skills CG7 and CE3.
The methodology of the equivalent course in the new curriculum, which offers face-to-face teaching, will be followed:
P4142107 - Metabolism and genetic engineering
The assessment system of the equivalent course in the new curriculum, which offers face-to-face teaching, will be followed:
P4142107 - Metabolism and genetic engineering
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Jaime Jose Gomez Marquez
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881816927
- jaime.gomez.marquez [at] usc.es
- Category
- Professor: University Professor
Cristina Diaz Jullien
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881816932
- cristina.diaz [at] usc.es
- Category
- Professor: Temporary PhD professor